174,565 results
-
-
pCMV-evoCAST-TniQ
Plasmid#234728PurposeEngineered human-codon optimized PseTniQ in mammalian cells (for evoCAST)DepositorInsertPseTniQ
ExpressionMammalianAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-mScarlet3_N1
Plasmid#189767PurposeConstruct for labelling actin filaments in mammalian cells with lifeact-mScarlet3DepositorInsertLifeAct-mScarlet3
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-Cre-4x6T
Plasmid#196410PurposeAstrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorHas ServiceAAV5InsertCre
UseAAV; Astrocyte-selectiveExpressionMammalianPromoterCAG and GfaABC1DAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FIRE-pHLy
Plasmid#170774PurposeRatiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterhUbCAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SRRM2-mCherry
Plasmid#174089PurposeInducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on systemDepositorAvailable SinceSept. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS023: Peredox NADH/NAD+ sensor (cytosolic)
Plasmid#163060PurposePeredox NADH/NAD+ sensor (cytosolic)DepositorInsertPeredox
UseLentiviralTagsmCherryExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8f-WPRE
Plasmid#162379PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8f
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationQ315LPromoterSynapsinAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xHA-TurboID_pAS31
Plasmid#118220Purposeexpresses 3xHA-tagged TurboID in C. elegans intestineDepositorInsertTurboID (BirA mutant)
Tags3x HA tagExpressionWormMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterges-1pAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx3
Plasmid#116855PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
TagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationamino acids 169-329 (tandem trimer)Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDB327
Plasmid#113671Purposeexpresses Ulp1_R3 in Escherichia coliDepositorInsertUlp1_R3
Tags6-HisExpressionBacterialMutationcontains solubility enhancing mutationsPromoterT7Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
OmpA3-PNGaseF-TEV-His6
Plasmid#114274PurposeAmidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.DepositorInsertPeptide: N-glycosidase F
TagsOmpA3 leader sequence (for periplasmic localisati…ExpressionBacterialPromoterT7Available SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm
Plasmid#63121Purposemini-Tn7 vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 17, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
C1(1-29)-miniTurbo-V5_pLX304
Plasmid#107176Purposeexpresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vectorDepositorInsertminiTurbo (BirA mutant)
UseLentiviralMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MBP-2xNLS-tdTomato
Plasmid#104054PurposeAn AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoterDepositorInsert2xNLS-tdTomato
UseAAVTagsNLSPromoterMBPAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-ASC1
Plasmid#75134PurposeExpresses N-terminal Flag-tagged ASC1 in mammalian cellsDepositorInsertPYD and CARD domain containing (Pycard Mouse)
TagsFLAGExpressionMammalianMutationF23S (please see depositor comments below)PromoterCMVAvailable SinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-wtYAP
Plasmid#84009Purposeexpresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertYAP1 (siRNA insensitive) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMammalianPromoterTRE-CMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ hnRNPK myc HIS
Plasmid#71662PurposeMammalian expression of hnRNPKDepositorAvailable SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_iSH2-pMag(3x)-iRFP
Plasmid#67304PurposeBlue light dependent photoswitching systemDepositorAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
5'UTR CGG 99x FMR1-EGFP
Plasmid#63091PurposeContains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.DepositorInsertexpanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only
