163,364 results

1. pSI-Check2-hRluc-NFkB-firefly

   Plasmid

   #106979

   Purpose

   Luciferase reporter with internal control for NFkB signal.

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   NFKB recognition sites (NFKB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUW-TetO-ASB2

   Plasmid

   #207185

   Purpose

   doxycycline-inducible expression of Human ASB2 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

   Insert

   pFUW-TetO-ASB2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAVEC1_C-mEGFP

   Plasmid

   #231279

   Purpose

   For stably expressing fusion proteins with C terminally tagged mEGFP in plants

   Depositor

   Jacob Brunkard

   Article

   Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

   Type

   Empty backbone

   Use

   Tags

   mEGFP

   Expression

   Plant

   Mutation

   N/A

   Promoter

   Available since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p300dAIL KAT LIC 1M

   Plasmid

   #233588

   Purpose

   Bacterial expression of p300 KAT enzyme with truncated autoinhibitory loop

   Depositor

   Scott Showalter

   Article

   Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

   Insert

   p300dAIL KAT (EP300 Human)
   

   Use

   Tags

   6xHis MBP

   Expression

   Bacterial

   Mutation

   deleted amino acids 1523-1554

   Promoter

   T7

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP

   Plasmid

   #234881

   Purpose

   L1HS-silencing plasmid (CRISPRi gRNA2)

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   LacZ gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

   Plasmid

   #234882

   Purpose

   L1HS-silencing plasmid (CRISPRi gRNA1)

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   L1HS-silencing plasmid (CRISPRi gRNA1)
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI5-CasRx-HA (pKW88)

   Plasmid

   #229046

   Purpose

   Mammalian expression of CasRx-HA

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Insert

   CasRx (RfxCas13d)
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGLO-Yellow-AZA

   Plasmid

   #220783

   Purpose

   Expresses a yellow chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)

   Depositor

   Angela Leu

   Insert

   yellow chromoprotein
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-mGold2t-CAF1-C-10

   Plasmid

   #231782

   Purpose

   Visualization of nucleus in mammalian cells using mGold2t

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2t-CAF1-C-10 (Chaf1a Mouse)
   

   Use

   Tags

   mGold2s

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-EF1A-Puro-hTBK1-E696K-EGFP

    Plasmid

    #221554

    Purpose

    Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Piggybac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed glutamate 696 to lysine

    Promoter

    EF1a

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCL1313

    Plasmid

    #217955

    Purpose

    Expression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC12

    Plasmid

    #197406

    Purpose

    Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pBBR1 replicon

    Depositor

    Yolanda Schaerli

    Article

    Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

    Insert

    pBBR1 replicon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Salsa-BC96-P1H12

    Plasmid

    #202937

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC96

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC96
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a MDHP_YEAST

    Plasmid

    #204233

    Purpose

    Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHP_YEAST (MDH3 Budding Yeast)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    The last 3 C-terminal residues (SKL) are the pero…

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE60 Q58L wgMDH

    Plasmid

    #204246

    Purpose

    Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Q58L

    Promoter

    T5

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD649-HAsp-AGER-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156761

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    AGER (AGER Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet-1_mNeongreen-Erythropoietin

    Plasmid

    #182422

    Purpose

    Bacterial expression of mNeongreen-Erythropoietin fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    mNeongreen-eythropoietin (EPO Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet-1_VNp-mNeongreen-Erythropoietin

    Plasmid

    #182423

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-eythropoietin (EPO Human)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2)

    Plasmid

    #185448

    Purpose

    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Inserts

    TRF2 (TERF2 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    

    Use

    Tags

    MBP, YBBR, and ZZ

    Expression

    Insect

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTS2355_Tier3(PB)-iRFP_PuroR

    Plasmid

    #169654

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven iRFP670 and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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