159,428 results

1. His6-pAG-RTase

   Plasmid

   #214932

   Purpose

   Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

   Depositor

   Chuan He

   Article

   Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

   Insert

   fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

   Promoter

   T7 promoter

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-Tre3G-29xGGGGCC-12xMS2

   Plasmid

   #99149

   Purpose

   Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   29xGGGGCC 12xMS2 hairpins
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

   Plasmid

   #141391

   Purpose

   Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-N (N SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 8, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLenti-CMV-Puro-AAVR-FLAG

   Plasmid

   #166716

   Purpose

   Vector expressing AAVR (KIAA0319L) with a C-terminal Flag tag

   Depositor

   Jan Carette

   Article

   Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

   Insert

   AAVR-FLAG (KIAA0319L Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ND4-DdCBE- left side TALE

   Plasmid

   #157844

   Purpose

   expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4xnrUAS:GFP

   Plasmid

   #61373

   Purpose

   4xnrUAS driving GFP

   Depositor

   Marnie Halpern

   Article

   Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

   Insert

   4xnr UAS:GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP

   Plasmid

   #55643

   Purpose

   vCre-Dependent ChR2-EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   hChR2(H134R)-EYFP
   

   Use

   AAV; Vcre-dependent chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

   Plasmid

   #141386

   Purpose

   Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-M (M SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 8, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCAG-Scarlet-Kif5a

   Plasmid

   #118745

   Purpose

   Expresses scarlet tagged Kif5a

   Depositor

   Beverly Koller

   Insert

   Kif5a cDNA (Kif5a Mouse)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4.TO-K5-2xCSTREP

    Plasmid

    #136154

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTD-NTwinStrep_Sm

    Plasmid

    #45937

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. TagBFP2-CAAX

    Plasmid

    #116856

    Purpose

    plasma membrane marker

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    HRAS
    

    Use

    Tags

    TagBFP2

    Expression

    Mammalian

    Mutation

    Amino acids 172-189

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5FRT/Flag-LOC339287(MSL1)

    Plasmid

    #29762

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    MSL1 (MSL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid

    #89873

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (Please see depositor comments …

    Promoter

    Human synapsin

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCherry-SopF

    Plasmid

    #135174

    Purpose

    Expresses an mCherry-SopF fusion in mammalian cells

    Depositor

    Leigh Knodler

    Article

    Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

    Insert

    SopF (STM1239 Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUb FLAG-Human IntS6

    Plasmid

    #198408

    Purpose

    Expresses FLAG-tagged human IntS6

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    DDX26 (INTS6 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-C-mScarlet-Zeo

    Plasmid

    #207733

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pMito-LSSmOrange

    Plasmid

    #37135

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

    Insert

    LSSmOrange
    

    Use

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    CMV

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ube2v1

    Plasmid

    #31429

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2V1 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pYESir2HY-puro

    Plasmid

    #1770

    Depositor

    Bob Weinberg

    Article

    Vaziri et al Cell 2001 Oct 19;107(2):149-59.

    Insert

    Sir2 (SIRT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H363Y, residue 363 changed from histidine to tyro…

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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