161,641 results

1. HDM_Spikedelta21_sinobiological_B.1.617.2

   Plasmid

   #204149

   Purpose

   Expression plasmid for SARS-CoV-2 Delta variant spike

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   SARS-CoV-2 Delta variant spike (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   21 amino acid cytoplasmic tail deletion

   Promoter

   CMV

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMApra-Ptac

   Plasmid

   #218357

   Purpose

   Empty vector with lacIq-Ptac expression system

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FU-H2B-GFP-IRES-Puro

   Plasmid

   #69550

   Purpose

   Lentiviral, selectable fluorescent GFP tagging of nuclei.

   Depositor

   Charles Gersbach

   Insert

   Histone 2B - green fluorescent protein fusion
   

   Use

   Lentiviral

   Tags

   GFP fusion

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mc2 mNeon

   Plasmid

   #206218

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LeGO-G

   Plasmid

   #27347

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   U6 promoter, SFFV promoter, eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1-TMEM192-FLAG

   Plasmid

   #164977

   Purpose

   Lentiviral vector for TMEM192 expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAKgfplux1

   Plasmid

   #14083

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Bacterial luciferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pR26 CAG AsiSI/MluI

   Plasmid

   #74286

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE

   Plasmid

   #21836

   Depositor

   David Ron

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   farnysylation signal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. phND2-N174

    Plasmid

    #31822

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    NeuroD2 (NEUROD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-CalfluxCTN

    Plasmid

    #190068

    Purpose

    Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

    Depositor

    Carl Johnson

    Article

    Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

    Insert

    CalfluxCTN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. SP6-VEE-OKS-iM

    Plasmid

    #58973

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGD248

    Plasmid

    #219498

    Purpose

    Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti-CAG -IRES-GFP

    Plasmid

    #69047

    Purpose

    Lentiviral backbone for GFP coexpression

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. G protein alpha-s-HaloTag

    Plasmid

    #66993

    Purpose

    G protein alpha-s internally tagged with HaloTag and EE epitope

    Depositor

    Catherine Berlot

    Insert

    G-alpha-s-EE-HaloTag (Gnas Rat)
    

    Use

    Tags

    HaloTag flanked by SGGGGSGGGGS on each side was i…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti PGK V5-LUC Puro (w543-1)

    Plasmid

    #19360

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. FL MALT1 WT in pET29b-Strep

    Plasmid

    #48968

    Purpose

    Expresses Full length wild type MALT1 in e.coli

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 isoform A (MALT1 Human)
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pTRANS_231

    Plasmid

    #91120

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF0113

    Plasmid

    #141520

    Purpose

    Lentiviral vector for overexpressing the SMAD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBTK800

    Plasmid

    #179209

    Purpose

    GFP dropout for assembling a double-stranded RNA expression plasmid

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

    Insert

    GFP dropout
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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