159,461 results
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Plasmid#128063PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only
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TFORF3138
Plasmid#144614PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPPARG (PPARG Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Cre
Plasmid#34564PurposeRetroviral expression of CreDepositorInsertCre
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailable sinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)
Plasmid#197510PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSExpressionMutationABE8e mutations in TadA and nSpRY(D10A/A61R)PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-mCD47-T2A-GFP
Plasmid#205449Purposelentiviral plasmid for expression of mouse CD47DepositorInsertmCD47 (Cd47 Mouse)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSin-EF2-Sox2-Pur
Plasmid#16577DepositorInsertHomo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
HP17_KO7
Plasmid#120346PurposeHelper plasmid for production of phagemid ssDNA, based on M13KO7DepositorInsertCoding part of the M13KO7 helper phage genome
UseTagsExpressionBacterialMutationPromoternative phage promotersAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP
Plasmid#50465PurposeEGFP under the control of human synapsin promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial sizeInsertEGFP
UseAAVTagsN/AExpressionMutationPromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_FLUBV_HA
Plasmid#207274PurposeMammalian expression of Influenza B virus HA proteinDepositorInsertFLUBV_HA (HA Influenza B virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKK-BiFC-Venus
Plasmid#105804PurposeConcomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.DepositorTypeEmpty backboneUseFlp-in competentTagsVN173 (I152L); VC155ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-AR Q0
Plasmid#86427PurposeFluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine regionDepositorInsertandrogen receptor (AR) (AR Human)
UseTagsEGFPExpressionMammalianMutationDeletion of the poly-glutamine expansionPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBbi G418 Hu CD19
Plasmid#183247PurposeExpression of human CD19 antigen in a Sleeping Beauty transposon vectorDepositorInsertCD19 (CD19 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-FGE
Plasmid#75147PurposeExpression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.DepositorInsertFGE
UseTagsHistidine tag and ThrombinExpressionBacterialMutationN/APromoterT7Available sinceJuly 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2027
Plasmid#143994PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNPAS2 (NPAS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH2.9_MMLV_Gag-P2A-eGFP
Plasmid#205524PurposeExpress MMLV Gag-P2A-eGFPDepositorInsertGag
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-GRAM-H
Plasmid#211700PurposeExpression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (EGFP-GRAM1b G187L) in mammalian cellsDepositorInsertGRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsEGFPExpressionMammalianMutationChanged Glycine 187 to LeucinePromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPSU40
Plasmid#173831PurposeExpresses 40 kD Penn State ladder proteinDepositorInsertHSTPABPACGST
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-F-del Casp9.IRES.GFP
Plasmid#15567DepositorInsertFKBP12(V36)-p30Caspase9 (FKBP1A Human)
UseRetroviralTagsFKBP12 and HA epitopeExpressionMammalianMutationFKBP12 F36 to V Truncated Caspase9 (135-416; lac…PromoterAvailable sinceAug. 22, 2007AvailabilityAcademic Institutions and Nonprofits only