179,805 results
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Plasmid#189771PurposeConstruct for labelling plasma membranes in mammalian cells using LCK-mScarlet3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLCK-mScarlet3
ExpressionMammalianPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
phCMV-coGALVenv-C4070A
Plasmid#172217PurposeExpresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectorsDepositorInsertEnvelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
ExpressionMammalianMutationCarries C-terminus of amphotropic MLV strain 4070…PromoterCMV immediate early promoterAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
scFLARE2
Plasmid#158700PurposeNeuronal expression of scFLARE2 componentDepositorInsertscFLARE2
UseAAVTagsHA, Flag, VP16ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterSynAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-S5E2-dTom-nlsdTom
Plasmid#135630PurposeAAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory elementDepositorHas serviceAAV1, AAV9, and AAV PHP.eBInsertdTom-nlsdTom
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PCR4-Shh::lacZ-H11
Plasmid#139098PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingExpressionMammalianPromoterShhAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EF1a_mCherry_P2A_Hygro
Plasmid#135003PurposeLentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ARR3tk-eGFP/SV40-mCherry
Plasmid#132360PurposeTo measure transcriptional activity of Androgen ReceptorDepositorInsertAR responsive elements (AR Human)
UseLentiviralAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-HA-NONO
Plasmid#127655PurposeOver-express HA-tagged human NONODepositorAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV/L7-6-GFP-WPRE
Plasmid#126462PurposeAn AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.DepositorInsertL7-6, Purkinje cell specific promoter
UseAAVExpressionMammalianAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-U6insert-EF1puro
Plasmid#104537PurposeA piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAsDepositorTypeEmpty backboneUseCRISPR, Mouse Targeting, and Synthetic Biology ; …ExpressionMammalianPromoterU6Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EGFP-BAF
Plasmid#101772PurposeExpresses EGFP tagged BAF in human cellsDepositorAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Plasmid#92392Purposeproduces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTADepositorInsertTM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
UseAAV and Synthetic BiologyExpressionMammalianPromoterHuman SynapsinAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
Tags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ca-FLARE (TF)
Plasmid#92213PurposeTranscription factor component of FLAREDepositorInsertNRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16
UseAAVExpressionMammalianPromotersynapsinAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJ117
Plasmid#85997PurposeOne-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3DepositorInsertsEGFP-NT2_cr3
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianPromotermodified human U6Available sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX601-mCherry
Plasmid#84039PurposeStaphylococcus aureus (SaCas9) conjugated with mCherryDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-mCherryExpressionMammalianPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
Plasmid#67979PurposeCas9 activity reporter (control) with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBluescriptSKII+ U6-sgRNA(F+E) empty
Plasmid#74707PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
ExpressionMammalianAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHRm-GFP(1-10)-VP64-NLS
Plasmid#70228PurposeExpresses GFP(1-10)-VP64-NLS in mammalian cellsDepositorInsertGFP(1-10)-VP64-NLS
UseLentiviralExpressionMammalianAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hNox4
Plasmid#69352Purposeexpresses human Nox4 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthNOX4 (NOX4 Human)
ExpressionMammalianPromoterCMVAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by