177,604 results

Showing: 4821 - 4840 of 177604 results

Results per page:

Sort results by:

PB-TO-NFIA.1-SOX9

Plasmid

#182310

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA-T2A-SOX9 (NFIA, SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Nsp3 -EGFP

Plasmid

#165108

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Publication

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nsp3 (ORF1ab SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
mCh-Mfn2

Plasmid

#141156

Purpose

Mammalian expression and labeling mitofusin 2

Depositor

Gia Voeltz

Publication

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mitofusin 2 (MFN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
8xNFAT-ZsG-hCD8

Plasmid

#153417

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

Depositor

Maki Nakayama

Publication

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )

Use

Retroviral

Expression

Mammalian

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Publication

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pcDNA3 TFAM-mScarlet

Plasmid

#129573

Purpose

Expression of mScarlet fused TFAM

Depositor

Stephen Tait

Publication

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pcDNA3.1-HA-NONO

Plasmid

#127655

Purpose

Over-express HA-tagged human NONO

Depositor

Nicolas Manel

Publication

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO (NONO Human)

Tags

HA

Expression

Mammalian

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

Plasmid

#126580

Purpose

A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Publication

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Tags

HA

Expression

Mammalian

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

Plasmid

#122151

Purpose

Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

dCas9 fused to SunTag(24x)

Use

Lentiviral

Tags

2xNLS-P2A-BFP and NLS

Expression

Mammalian

Promoter

SFFV

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pHAGE-BRAF-V600E

Plasmid

#116204

Purpose

Lentiviral expression of BRAF V600E

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600E

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
PB-U6insert-EF1puro

Plasmid

#104537

Purpose

A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Publication

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Expression

Mammalian

Promoter

U6

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
LbCpf1-2NLS

Plasmid

#102566

Purpose

bacterial expression of LbCpf1-2NLS

Depositor

Jennifer Doudna

Publication

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

LbCpf1

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Promoter

T7

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

Enlarge
pX330-EN1201

Plasmid

#92144

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus

Expression

Mammalian

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

Enlarge
6-His-MBP-TEV-FnCpf1

Plasmid

#90094

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Publication

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 (humanized)

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Promoter

T7

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pU6-Cj-sgRNA

Plasmid

#89753

Purpose

U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

Depositor

Seokjoong Kim

Publication

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cj-SgRNA

Use

Sgrna expression under u6 promoter

Promoter

U6

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

Plasmid

#85452

Purpose

Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

Depositor

Aviv Regev

Publication

Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

Insert

U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Publication

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Promoter

CAG

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

Enlarge
TrkB mEGFP

Plasmid

#83952

Purpose

FRET donor for dynamically measuring TrkB activity in neurons

Depositor

Ryohei Yasuda

Publication

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tropomyosin related kinase B (Ntrk2 Mouse)

Tags

mEGFP

Expression

Mammalian

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pCDNA3 HA C9ORF72 op

Plasmid

#74156

Purpose

Express HA tagged optimized C9ORF72 in mammalian cells

Depositor

Nicolas Charlet-Berguerand

Insert

C9ORF72 (C9orf72 Synthetic, Human)

Tags

HA

Expression

Mammalian

Mutation

optimized sequence

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
Cas9-NAT

Plasmid

#64329

Purpose

Cas9 expression cassette carried by a yeast single-copy episome plasmid

Depositor

Yong-Su Jin

Publication

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette

Use

CRISPR

Expression

Yeast

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

Enlarge