165,744 results

1. 10279-M28-663

   Plasmid

   #225662

   Purpose

   Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

   Depositor

   Dominic Esposito

   Article

   Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

   Insert

   dsCopGFP
   

   Use

   Lentiviral

   Promoter

   mCMVp

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

   Plasmid

   #31484

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   EF1s HA NLS Sce(opt) T2A IFP1.4
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Mutation

   "Opt" indicates the I-SceI coding seque…

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Bcl2

   Plasmid

   #195551

   Purpose

   Expresses Bcl2 in mammalian cells

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Bcl2 (Bcl2 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CMV enhancer and promoter

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. sfGFP-Peroxisomes-2

   Plasmid

   #54601

   Purpose

   Localization: Peroxisomes, Excitation: 485, Emission: 507

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   Peroximal Targeting Signal 1 (PTS1) and sfGFP

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pHAGE-TEX264-GFP

   Plasmid

   #201925

   Purpose

   For lentiviral transduction of TEX264-GFP

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-TEX264ΔLIR-GFP

   Plasmid

   #201926

   Purpose

   For lentiviral transduction of TEX264ΔLIR-GFP

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Lentiviral

   Mutation

   F273A

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDAC565

   Plasmid

   #195242

   Purpose

   Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Caspase1-flag

   Plasmid

   #21142

   Depositor

   Junying Yuan

   Article

   Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

   Insert

   caspase-1 (Casp1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCD-GSDMA-N-tev-C

   Plasmid

   #226231

   Purpose

   Express TEVP-cleavable GSDMA

   Depositor

   Michael Elowitz

   Article

   Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

   Insert

   Gasdermin A (Gsdma3 Mouse)
   

   Expression

   Mammalian

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-nSpCas9-TadA8e

    Plasmid

    #209044

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TadA8e-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLH01

    Plasmid

    #117261

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    PorfX-Lp_0640-42, sapK-sappR
    

    Use

    Other

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-DN-hCUL2-FLAG

    Plasmid

    #15819

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin2 (CUL2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N427

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCWXPGR-pTF-Kir2.1

    Plasmid

    #114284

    Purpose

    TET-inducible expression of the Kir2.1 protein

    Depositor

    Patrick Salmon

    Insert

    Kir2.1 (KCNJ2 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-myc-PDGFRbeta

    Plasmid

    #136455

    Purpose

    Mammalian expression plasmid for c-myc-tagged PDGFRB

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRbeta (PDGFRB Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2-10xUAS-Voltron

    Plasmid

    #119039

    Purpose

    UAS-driven expresion of Voltron in zebrafish

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    Zebrafish expression

    Promoter

    E1b

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGADT7-GW

    Plasmid

    #61702

    Purpose

    Gateway compatible Y2H vector - AD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. E326K GBA pcDNA3.1

    Plasmid

    #188581

    Purpose

    Ectopic expression E326K GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Expression

    Mammalian

    Mutation

    E326K

    Promoter

    CMV

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-SARS2-Spike

    Plasmid

    #145032

    Purpose

    Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 Spike (S Synthetic)
    

    Tags

    C9

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLCKOv3

    Plasmid

    #209046

    Purpose

    Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMH0006

    Plasmid

    #135448

    Purpose

    Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.

    Depositor

    Jonathan Weissman, Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    dCas9-BFP-KRAB
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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