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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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161,641 results

Showing: 4881 - 4900 of 161641 results

  2. pSV40-mCherry-P2A-Hygro

    Plasmid
    #106478
    Purpose
    Expression of mCherry and Hygromycin resistance gene from SV40 promoter
    Depositor
    Alex Hewitt
    Inserts
    mCherry
    Hygromycin B resistance
    Use
    Tags
    Expression
    Mammalian and Plant
    Mutation
    Promoter
    SV40 promoter
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106478-plasmid-map-sequence-id-207986

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  5. FUGW-APOE-E4-T2A-mCherry

    Plasmid
    #190809
    Purpose
    Express APOE E4 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E4
    Promoter
    Ubi C
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190809-plasmid-map-sequence-id-374463

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  7. mApple-LAMP1-phLuorin-N-8

    Plasmid
    #54918
    Purpose
    Localization: Lysosome Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    LAMP1 phLuorin
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Lysosomes-20 double labeled with phLuorin
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54918-plasmid-map-sequence-id-372787

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  9. GFP-C1-PLCdelta-PH

    Plasmid
    #21179
    Purpose
    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids
    Depositor
    Tobias Meyer
    Article
    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.
    Insert
    PLCdelta (PLCD1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    PH domain of human PLC delta, amino acids Met1–Il…
    Promoter
    Available since
    July 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21179-plasmid-map-sequence-id-212104

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  10. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  15. pUC57-TPNOX

    Plasmid
    #87853
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    TPNOX
    Use
    Tags
    C-terminal FLAG tag with a linker
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87853-plasmid-map-sequence-id-175126

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  16. TFORF0985

    Plasmid
    #142249
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF4 (KLF4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    142249-plasmid-map-sequence-id-385602

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  20. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid
    #85452
    Purpose
    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
    Depositor
    Aviv Regev
    Article
    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do
    Insert
    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    85452-plasmid-map-sequence-id-173969

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Showing: 4881 - 4900 of 161641 results