159,352 results

1. AICSDP-26:ST6GAL1-mEGFP

   Plasmid

   #101786

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1

   Depositor

   Allen Institute for Cell Science

   Insert

   ST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. Adeno EA

   Plasmid

   #64071

   Purpose

   Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Insert

   U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
   

   Use

   Adenoviral

   Tags

   FLAG-Cas9

   Expression

   Mutation

   Promoter

   U6 and CBh

   Available since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pMRE162

   Plasmid

   #118510

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLminP_Luc2P_RE57

   Plasmid

   #90402

   Purpose

   Type I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   GR

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIRES2-EGFP-SHP2WT

   Plasmid

   #12283

   Depositor

   Anton Bennett

   Article

   Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

   Insert

   SHP-2WT (PTPN11 Human)
   

   Use

   Tags

   Myc (not canonical: KLISEEDL)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL3-cMYC promoter

   Plasmid

   #114724

   Purpose

   reporter luciferase construct

   Depositor

   Martine Roussel

   Article

   Roussel et al Oncogene. 1994 Feb;9(2):405-15.

   Insert

   C-Myc promoter region
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

   Plasmid

   #175573

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmTurquoise2-Golgi

   Plasmid

   #36205

   Purpose

   In vivo visualization of golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   Golgi targeting sequence (B4GALT1 Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

   Plasmid

   #208696

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3m
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SIVu

    Plasmid

    #81089

    Purpose

    Single Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AiP12109 - pAAV-AiE0410m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2109)

    Plasmid

    #208157

    Purpose

    Direct-expressing SYFP2 in oligodendrocytes AAV Virus

    Depositor

    Boaz Levi, The Allen Institute for Brain Science

    Article

    Mich et al bioRxiv 2023.09.20.558718

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR-HNRNPA1C-mCh-Cry2WT

    Plasmid

    #101226

    Purpose

    hnRNPA1(186-320) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    hnRNPA1(186-320) (HNRNPA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKRaION2-N1

    Plasmid

    #177802

    Purpose

    Mammalian expression of green fluorescent potassium indicator KRaION2

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-CRY2-mCherry

    Plasmid

    #58368

    Purpose

    Mamlian codon optimized CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mPiezo2-CMV-Sport6

    Plasmid

    #81073

    Purpose

    mouse Piezo2

    Depositor

    Ardem Patapoutian

    Article

    Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

    Insert

    Piezo2 (please see depositor comments below) (Piezo2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pInducer20_EGFP

    Plasmid

    #228878

    Purpose

    Tet-inducible lentiviral vector for expression of optimized GFP

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a-GluRS-His

    Plasmid

    #124109

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutamyl-tRNA ligase (gltX Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAW63.YY1.FKBP.knock-in.BFP

    Plasmid

    #104371

    Purpose

    For knocking in FKBP F36V P2A BFP

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    FKBP12(F36V) -P2A-BFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tetO-TBX20

    Plasmid

    #170705

    Purpose

    doxycycline-inducible overexpression of TBX20

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    TBX20 (TBX20 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ai162 (TIT2L-GC6s-ICL-tTA2) targeting vector

    Plasmid

    #114433

    Purpose

    Target a Cre-dependent GCaMP6s cassette and a tTA2 cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GCaMP6s, tTA2
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    TRE2, CAG

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge