170,492 results

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pmStayGold_mStayGold_I3

Plasmid

#239339

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mStayGold

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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CTCF-mAC donor (Neo)

Plasmid

#140645

Purpose

CTCF tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3 1kb prom. CD274

Plasmid

#107002

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-UBR5

Plasmid

#231238

Purpose

Flag-tagged wild-type UBR5 over-expression construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

UBR5 (UBR5 Human)

Expression

Mammalian

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-dTAG

Plasmid

#207694

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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tFucci(SA)5

Plasmid

#153520

Purpose

New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

Depositor

Atsushi Miyawaki

Article

Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

Insert

tFucci(SA)5.

Tags

AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK-GFP.WPRE

Plasmid

#12252

Purpose

3rd generation lentiviral backbone

Depositor

Didier Trono

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-YFP

Plasmid

#13033

Purpose

Mammalian expression vector for cloning your gene fused to YFP

Depositor

Doug Golenbock

Has Service

Cloning Grade DNA

Insert

Yellow fluorescent protein

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-ExRai-AktAR2

Plasmid

#184050

Purpose

Plasma membrane-targeted ExRai-AktAR2

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

PM-ExRai-AktAR2

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Myo10

Plasmid

#135403

Purpose

Expresses GFP-tagged bovine Myo10 in mammalian cells.

Depositor

Richard Cheney

Article

Berg et al Nat Cell Biol. 2002 Mar;4(3):246-50. doi

Insert

Myo10 (MYO10 Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

"Relative to the GenBank Myo10 cDNA sequence…

Promoter

CMV

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-Q23

Plasmid

#40261

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q23 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS2-Spike

Plasmid

#145032

Purpose

Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Article

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

SARS-CoV-2 Spike (S Synthetic)

Tags

C9

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-HA-hG9a

Plasmid

#33024

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pNCS-Antares

Plasmid

#74279

Purpose

Constitutive expression of Antares in bacteria

Depositor

Michael Lin

Article

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Antares

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Hygro-dTomato

Plasmid

#99376

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-dTomato

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDN34-BiNLS-hYAP1-1delta

Plasmid

#169471

Purpose

expresses optoYAP in mammalian cells

Depositor

Timothy Saunders

Article

Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

Insert

YAP1-1delta (YAP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EGFP-Cre

Plasmid

#86805

Purpose

Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter

Depositor

Amy Lasek

Article

Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02

Insert

Cre recombinase

Use

Cre/Lox and Lentiviral

Tags

EGFP and nuclear localization signal: PKKKRKV

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP

Plasmid

#225446

Purpose

Express mEGFP-fusion protein of interest

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Type

Empty backbone

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pKP332 (Lenti-OSK1)

Plasmid

#21627

Depositor

Tim Townes

Article

Chang et al Stem Cells. 2009 May . 27(5):1042-9.

Insert

hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Human, Porcine teschovirus-1)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Nov. 12, 2009

Availability

Academic Institutions and Nonprofits only

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