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pAd PdxI-I-nGFP

Plasmid

#19411

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

pancreatic and duodenal homeobox 1 (Pdx1 Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 barr1 HA

Plasmid

#14693

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta arrestin 1 (Arrb1 Rat)

Tags

HA

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pHes1-GFPd2

Plasmid

#14808

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Hes1 promoter

Tags

GFPd2

Expression

Mammalian

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0-HA-UbcH13

Plasmid

#12461

Depositor

Dong-Er Zhang

Article

Zou et al Biochem Biophys Res Commun. 2005 Oct 14.

Insert

Ubc13 (UBE2N Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pJ3M-Mst2

Plasmid

#12205

Depositor

Jonathan Chernoff

Article

Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

Insert

Mst2 (STK3 Human)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-2T PTP-1B

Plasmid

#8602

Depositor

Ben Neel

Insert

PTP-1B (PTPN1 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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L4440gtwy

Plasmid

#11344

Depositor

Guy Caldwell

Insert

gateway cassette

Use

RNAi

Expression

Worm

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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Ub-G76V-YFP

Plasmid

#11949

Depositor

Nico Dantuma

Article

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

Ub-G76V-YFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of YFP. Glycine 76 …

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf2 C716A

Plasmid

#11747

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Tags

Flag

Expression

Mammalian

Mutation

C716A: catalytically inactive

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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Human Integrin beta 2

Plasmid

#8640

Depositor

Timothy Springer

Article

Kishimoto et al Cell 1987 Feb 27;48(4):681-90.

Insert

Integrin beta 2 (ITGB2 Human)

Expression

Mammalian

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.3_SARS2_omicron BA.2

Plasmid

#183700

Purpose

expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

c-terminal 18 aa deletion

Promoter

CMV

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron_BA.1

Plasmid

#180375

Purpose

Pseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike protein

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (omicron BA.1 variant) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

c terminal D18

Promoter

CMV

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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phCMV-coGALVenv-C4070A

Plasmid

#172217

Purpose

Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors

Depositor

Boris Fehse

Article

Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814

Insert

Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)

Expression

Mammalian

Mutation

Carries C-terminus of amphotropic MLV strain 4070…

Promoter

CMV immediate early promoter

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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Watermelon backbone

Plasmid

#155258

Purpose

Can be used to generate a Watermelon library

Depositor

Joan Brugge, Aviv Regev

Article

Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

Plasmid

#171588

Purpose

Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

Bxb1 integrase
iCasp9
BFP
rtTA3G

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pABE8e-protein

Plasmid

#161788

Purpose

Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

Depositor

David Liu

Article

Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

Insert

ABE8e base editor

Tags

8xHIS and BPNLS

Expression

Bacterial

Promoter

pRhaBAD

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

Plasmid

#167937

Purpose

Inducible CRISPRa expression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

dxCas9(3.7)-VPR

Use

Lentiviral

Promoter

tetON

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1765 (pSPIN-R, R*MmeI)

Plasmid

#160734

Purpose

Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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ARR3tk-eGFP/SV40-mCherry

Plasmid

#132360

Purpose

To measure transcriptional activity of Androgen Receptor

Depositor

Charles Sawyers

Article

Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

Insert

AR responsive elements (AR Human)

Use

Lentiviral

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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