165,744 results

1. pcDNA3.1(+)IRES GFP

   Plasmid

   #51406

   Purpose

   Expresses GFP from internal ribosome entry sequence, cloning component

   Depositor

   Kathleen_L Collins

   Article

   Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

   Insert

   IRES GFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.3_SARS2_JN.1

   Plasmid

   #218678

   Purpose

   expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (JN.1)
   

   Expression

   Mammalian

   Mutation

   L455S on the basis of BA.2.86

   Promoter

   CMV

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Anti-TARPGamma2/Stargazin [N245/1R]

   Plasmid

   #177521

   Purpose

   Mammalian Expression Plasmid of anti-TARPGamma2/Stargazin (Rat). Derived from hybridoma N245/1.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-TARPGamma2/Stargazin (Rattus norvegicus) recombinant mouse monoclonal antibody (Cacng2 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. IDG_KCND1_OE_1

   Plasmid

   #161640

   Purpose

   Overexpresses KCND1

   Depositor

   Mike McManus

   Insert

   KCND1 (KCND1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLAMP1-emiRFP670

   Plasmid

   #136570

   Purpose

   LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   LAMP1-emiRFP670
   

   Tags

   emiRFP670

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLM-CMV-R-Cre

   Plasmid

   #27546

   Purpose

   Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

   Insert

   mCherry_P2A_Cre recombinase
   

   Use

   Cre/Lox and Lentiviral

   Promoter

   CMV

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAM-AAV-mSncg-0.27kb-EGFP

   Plasmid

   #153185

   Purpose

   Truncated mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAVS1-iPEmax-Hygro

   Plasmid

   #214020

   Purpose

   inducible PEmax system for controllable prime editing; this plasmid is used to insert PEmax prime editor in one allele of AAVS1 locus

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PEmax
   

   Expression

   Mammalian

   Promoter

   TRE-tight

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pESR1.1.0-gDNA

   Plasmid

   #112433

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1

   Depositor

   Kevin White

   Insert

   ESR1 (ESR1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Rtn4a-mNeonGreen

    Plasmid

    #169032

    Purpose

    Express human Rtn4a

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rtn4a (RTN4 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SPLICSFAST-ER-MT-P2A

    Plasmid

    #214892

    Purpose

    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-ER-MT-P2A
    

    Tags

    none

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FUGW-d2GFP-ZEB1 3'UTR

    Plasmid

    #79601

    Purpose

    FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    d2GFP-Zeb1 3'UTR
    

    Use

    Lentiviral

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOpen-MMLV_RT (lack H)

    Plasmid

    #165556

    Purpose

    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pBS mPrx1 (CT#640)

    Plasmid

    #13961

    Depositor

    Cliff Tabin

    Article

    Logan et al Genesis. 2002 Jun . 33(2):77-80.

    Insert

    Prx1 promoter (Prrx1 Mouse)
    

    Expression

    Bacterial

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

15. mEmerald-Myopalladin-C-14

    Plasmid

    #54188

    Purpose

    Localization: Cytoskeleton , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Myopalladin (MYPN Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. mEos4a-Actin-C-18

    Plasmid

    #57488

    Purpose

    Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Insert

    Actin (ACTB Human)
    

    Tags

    mEos4a

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pHAGE-XPO1-R749Q

    Plasmid

    #116709

    Purpose

    Lentiviral expression of XPO1 R749Q

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    XPO1 (XPO1 Human)
    

    Use

    Lentiviral

    Mutation

    R749Q

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDIRECT_26G

    Plasmid

    #91149

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HRE:GFP-pGK:Cre

    Plasmid

    #68552

    Purpose

    Lentivirus expressing hypoxia-responsive GFP reporter and Cre

    Depositor

    Tyler Jacks

    Inserts

    Green Fluorescent Protein
    Cre
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    HRE and pGK

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CDK2 gRNA (BRDN0001148950)

    Plasmid

    #77193

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK2 (CDK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge