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MMTV-SV40-Bssk

Plasmid

#1824

Depositor

Philip Leder

Publication

Ornitz et al Proc Natl Acad Sci U S A 1991 Feb 1;88(3

Type

Empty backbone

Expression

Mammalian

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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L4440gtwy

Plasmid

#11344

Depositor

Guy Caldwell

Insert

gateway cassette

Use

RNAi

Expression

Worm

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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Ub-G76V-YFP

Plasmid

#11949

Depositor

Nico Dantuma

Publication

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

Ub-G76V-YFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of YFP. Glycine 76 …

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-evoCAST-eCas7

Plasmid

#234726

Purpose

Engineered human-codon optimized PseCas7 in mammalian cells (for evoCAST)

Depositor

David Liu

Publication

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ePseCas7

Expression

Mammalian

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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PIEZO1-HaloTag

Plasmid

#207834

Purpose

PIEZO1 fusion for biochemistry and live imaging

Depositor

Charles Cox

Publication

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

PIEZO1 (PIEZO1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pK18msB

Plasmid

#177839

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

Depositor

Christopher Johnson

Publication

Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

Type

Empty backbone

Use

Bacterial gene replacement

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHage2-EF1a-TERT-IRES-Puro

Plasmid

#182590

Purpose

expresses TERT in mammalian cells

Depositor

Agnel Sfeir

Publication

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTERT (TERT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TO-NFIA.1-SOX9

Plasmid

#182310

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA-T2A-SOX9 (NFIA, SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron BA.2

Plasmid

#183700

Purpose

expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

Depositor

David Nemazee

Publication

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

c-terminal 18 aa deletion

Promoter

CMV

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron_BA.1

Plasmid

#180375

Purpose

Pseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike protein

Depositor

David Nemazee

Publication

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (omicron BA.1 variant) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

c terminal D18

Promoter

CMV

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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phCMV-coGALVenv-C4070A

Plasmid

#172217

Purpose

Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors

Depositor

Boris Fehse

Publication

Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814

Insert

Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)

Expression

Mammalian

Mutation

Carries C-terminus of amphotropic MLV strain 4070…

Promoter

CMV immediate early promoter

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pABE8e-protein

Plasmid

#161788

Purpose

Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

Depositor

David Liu

Publication

Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

Insert

ABE8e base editor

Tags

8xHIS and BPNLS

Expression

Bacterial

Promoter

pRhaBAD

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

Plasmid

#167937

Purpose

Inducible CRISPRa expression

Depositor

Emma Rawlins

Publication

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

dxCas9(3.7)-VPR

Use

Lentiviral

Promoter

tetON

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Mfn1

Plasmid

#141154

Purpose

Mammalian expression and labeling mitofusin 1

Depositor

Gia Voeltz

Publication

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 1 (MFN1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Publication

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLifeAct-HyPer7

Plasmid

#136464

Purpose

Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

Depositor

Vsevolod Belousov

Publication

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

LifeAct

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-APOBEC1-YTHmut

Plasmid

#131637

Purpose

Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

Depositor

Kate Meyer

Publication

Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

Insert

APOBEC1-YTHmut-HA (YTHDF2 Rat, Human)

Tags

HA

Expression

Mammalian

Mutation

YTH domain lacks AA 385-409 comprising the m6A-bi…

Promoter

CMV

Available since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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ARR3tk-eGFP/SV40-mCherry

Plasmid

#132360

Purpose

To measure transcriptional activity of Androgen Receptor

Depositor

Charles Sawyers

Publication

Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

Insert

AR responsive elements (AR Human)

Use

Lentiviral

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBT375

Plasmid

#125616

Purpose

Expresses evoAPOBEC1-BE4max-NG in mammalian cells

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max-NG

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh2.0

Plasmid

#106073

Purpose

express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

Depositor

Yulong Li

Publication

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 2.0

Expression

Mammalian

Promoter

CMV

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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