163,445 results

1. pSL21-VEX

   Plasmid

   #158230

   Purpose

   Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)

   Depositor

   Junwei Shi

   Article

   Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-U6-UL29-egfp-U3-UL8

   Plasmid

   #166694

   Purpose

   To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

   Depositor

   Yujia Cai

   Article

   Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

   Insert

   UL8 and UL29 gRNA, EGFP (UL8, UL29 HSV-1)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPS2037

   Plasmid

   #1370

   Depositor

   Pamela Silver

   Article

   Brodsky et al RNA 2000 Dec;6(12):1737-49.

   Insert

   PGK1-U1A-PGK1 3' UTR (PGK1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pLEX-TCRb-FKBP-Blasticidin

   Plasmid

   #120723

   Purpose

   Expresses ER-targeted recruiter protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   T cell receptor β chain
   

   Use

   Lentiviral

   Tags

   FKBP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. mClY-N1

   Plasmid

   #90457

   Purpose

   A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

   Insert

   mClY-N1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-U6-DR30(SapI)-EFS-CasRx-pA

   Plasmid

   #192488

   Purpose

   To express CasRX and a gRNA

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   CasRx
   

   Use

   AAV

   Tags

   Expression

   Mutation

   No

   Promoter

   EFS

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-Osp.gRNA-MS2in

   Plasmid

   #229772

   Purpose

   Contains the U6 promoter and an optimized gRNA backbone inserted with two copies of the MS2 stem loop.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

   Insert

   gRNA cassette with two MS2 stem loops
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-2NLS-Cas9

   Plasmid

   #229773

   Purpose

   Expresses Cas9 driven by a CMV promoter

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. DVK_pBR322_AE

   Plasmid

   #217592

   Purpose

   Destination vector with kanamycin resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-pcDNA3.1 Puro-CAG-Positron2

    Plasmid

    #239079

    Purpose

    Mammalian expression of positive-going voltage sensor

    Depositor

    GENIE Project

    Article

    Eric R. et al Janelia Figshare 2023

    Insert

    Positron2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R78K N81D D92N W178F

    Promoter

    CAG

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tad-CBE(GCC3)

    Plasmid

    #235078

    Purpose

    Expresses Tad-CBE(GCC3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(GCC3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tad-CBE(GCG4)

    Plasmid

    #235080

    Purpose

    Expresses Tad-CBE(GCG4) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(GCG4)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tad-CBE(ACC2)

    Plasmid

    #235061

    Purpose

    Expresses Tad-CBE(ACC2) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACC2)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-CAG-dCas9-VPR-pgk-hph

    Plasmid

    #233066

    Purpose

    Piggybac CRISPRa construct

    Depositor

    Markus Landthaler

    Article

    Vučićević et al bioRxiv 2024.09.12.612198

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sc RFC5-pCDFDuet

    Plasmid

    #239202

    Purpose

    Overexpress Sc RFC5 in E. coli

    Depositor

    Michael O'Donnell

    Insert

    RFC5 (RFC5 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. 8061-E31

    Plasmid

    #234268

    Purpose

    Gateway ORF Entry clone of mouse Smad1 with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    Smad1 (Smad1 Mouse)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBX_RPL7a_smRuby2_HA

    Plasmid

    #236087

    Purpose

    Constitutively encodes RPL7a-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting the 60S subunit.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    RPL7a-smRuby2-HA (RPL7A Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAIP4

    Plasmid

    #233686

    Purpose

    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.

    Depositor

    Christopher Bahl

    Insert

    Smt3 (SMT3 )
    

    Use

    Tags

    HisTag

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pEW108

    Plasmid

    #232823

    Purpose

    Expresses yeast compass complex in insect cells

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
    

    Use

    Tags

    His6-3xFLAG, Twinstrep

    Expression

    Insect

    Mutation

    Set1(762-1080)

    Promoter

    polyhedrin promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAIP4_9C74

    Plasmid

    #234238

    Purpose

    Heterologous protein expression of variant of super folded GFP in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    9C74
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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