165,528 results

1. TFORF1062

   Plasmid

   #143751

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFATC1 (NFATC1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mVenusC-Cavin3

   Plasmid

   #108890

   Purpose

   Expression of Cavin3 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   Cavin3 (altered codon usage) (CAVIN3 Human, Synthetic)
   

   Tags

   mVenus156-239

   Expression

   Mammalian

   Mutation

   altered codon usage

   Available Since

   March 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAF0689 Ef1a-Pa01_attB-BFP acceptor

   Plasmid

   #193472

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-Integrin-Alpha2-N-18

   Plasmid

   #55063

   Depositor

   Michael Davidson

   Insert

   Integrin-Alpha2 (ITGA2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_AncBE4max_P2A_GFP

   Plasmid

   #112100

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   AncBE4max_P2A_GFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

   Plasmid

   #164458

   Purpose

   AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   SYFP2
   

   Use

   AAV

   Promoter

   minCMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+ EB3-mKate2

   Plasmid

   #105940

   Purpose

   in vitro transcription

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   EB3 (MAPRE3 Human)
   

   Tags

   mKate2

   Expression

   Bacterial

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Capri(1-10)

   Plasmid

   #165142

   Purpose

   Expresses Capri(1-10) in mammalian cells

   Depositor

   Daichi Kamiyama

   Article

   Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

   Insert

   Capri(1-10)
   

   Expression

   Mammalian

   Available Since

   April 8, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. OMM-SPLICSs-nFAST

   Plasmid

   #210430

   Purpose

   Expresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sites

   Depositor

   Tito Calì

   Insert

   OMM-SPLICSs-nFAST
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF0658

    Plasmid

    #143722

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CHD3 (CHD3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223-CRKL

    Plasmid

    #23354

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CRKL (CRKL Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. His6-pAG-RTase

    Plasmid

    #214932

    Purpose

    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

    Depositor

    Chuan He

    Article

    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

    Insert

    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

    Promoter

    T7 promoter

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-tag

    Plasmid

    #55180

    Purpose

    FLAG-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    FLAG
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH47781

    Plasmid

    #48005

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pmEmerald-ERGIC-53

    Plasmid

    #170717

    Purpose

    Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    ERGIC-53 (LMAN1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCh-Mfn2

    Plasmid

    #141156

    Purpose

    Mammalian expression and labeling mitofusin 2

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Mitofusin 2 (MFN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mVenusN-Cavin3

    Plasmid

    #108889

    Purpose

    Expression of Cavin3 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    Cavin3 (altered codon usage) (CAVIN3 Human, Synthetic)
    

    Tags

    mVenus1-155

    Expression

    Mammalian

    Mutation

    altered codon usage

    Available Since

    March 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB2193

    Plasmid

    #67542

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAD29_hIRG1_4-461_pvp008

    Plasmid

    #124843

    Purpose

    Expresses human CAD (Irg1) in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-3 and 462-end.

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-ZIP(WT)

    Plasmid

    #27134

    Depositor

    Ron Vale

    Article

    Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

    Insert

    CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    eGFP and mCherry

    Expression

    Mammalian

    Available Since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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