165,528 results

1. CAG sHRPa-NRX

   Plasmid

   #73143

   Purpose

   Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-NRX
   

   Tags

   2x HA tag and Flag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-HA C/EBP gamma

   Plasmid

   #15715

   Depositor

   Joan Massague

   Article

   Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

   Insert

   C/EBP gamma (CEBPG Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

3. CAG sHRPb-NLG

   Plasmid

   #73144

   Purpose

   Small fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPb-NLG (Nlgn1 Mouse, Synthetic)
   

   Tags

   V5 tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-RFP

   Plasmid

   #22910

   Depositor

   Edward Callaway

   Article

   Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

   Insert

   Chicken Beta-actin promoter driving DsRedExpress
   

   Use

   AAV; Adeno-associated virus

   Expression

   Mammalian

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRU5HT1-inLuc

   Plasmid

   #58954

   Purpose

   HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

   Depositor

   David Derse, Dmitriy Mazurov

   Article

   Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

   Insert

   inLuc
   

   Use

   Retroviral

   Promoter

   CMV/R/U5

   Available Since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF3472

   Plasmid

   #144948

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFATC3 (NFATC3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPLICS PM-ER Long P2A

   Plasmid

   #164111

   Purpose

   Detect the long Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Long P2A
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK436 (mAID-mCherry-P2A-Hygro)

   Plasmid

   #168231

   Purpose

   C-terminal tagging with mAID-mCherry

   Depositor

   Masato Kanemaki

   Article

   Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

   Insert

   mAID-mCherry-P2A-Hygro
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSYC-437

   Plasmid

   #163829

   Purpose

   Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

   Depositor

   Seok-Yong Choi

   Article

   Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

   Inserts

   Foxj1a Promoter
   GCaMP6s
   

   Use

   Multisite gateway destination vector

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-Stx17

    Plasmid

    #45911

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    Syntaxin 17 (STX17 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAIP-hXRCC1pd-eGFP

    Plasmid

    #206033

    Purpose

    Mammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    hXRCC1
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

    Promoter

    CAG

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB CAG SMARCA4-IRES-EGFP

    Plasmid

    #153948

    Purpose

    piggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFP

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-CD36-C-10

    Plasmid

    #55011

    Purpose

    Localization: Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD36 (CD36 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid

    #219964

    Purpose

    Express human BCR-ABL1-P190 in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    BCR-ABL1-P190 (ABL1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORF2

    Plasmid

    #206026

    Purpose

    An expression vector for mouse LINE1 ORF2

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    ORF2 derived from mouse LINE1
    

    Expression

    Mammalian

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCC001

    Plasmid

    #124451

    Purpose

    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    HH-BsaI
    

    Expression

    Yeast

    Mutation

    gRNA expression cassette from pUDP002 modified to…

    Promoter

    TDH3 promoter from S.cerevisiae (already present …

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSC142

    Plasmid

    #122835

    Purpose

    SUC2 promoter with GreenGate A-B flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    SUC2 promoter
    

    Use

    Golden gate compatible cloning vector

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_SFFv_4D5-High-CAR_RHL002

    Plasmid

    #164825

    Purpose

    Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mscv2.2-6mycFlaA

    Plasmid

    #60203

    Purpose

    6xMYC-tagged full-length FlaA from Legionella pneumophila, expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of FlaA

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Insert

    FlaA
    

    Use

    Retroviral

    Tags

    6xMYC

    Expression

    Mammalian

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. hZMPSTE24-3FLAG_pQCXIP

    Plasmid

    #89759

    Purpose

    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

    Depositor

    Martin Dorf

    Article

    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

    Insert

    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    

    Use

    Retroviral; Bicistronic expression: puromycin re…

    Tags

    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

    Expression

    Mammalian

    Mutation

    Y253H in ZMPSTE24 (see depositor comments below)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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