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pEpicVector3mC_Puro-T2A-mCherry-ProtC-HA-FLAG

Plasmid

#162115

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to ProtC-HA-FLAG

Use

Lentiviral

Tags

ProtC-HA-FLAG

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-FLAG

Plasmid

#162086

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-HA-FLAG

Use

Lentiviral

Tags

VSVg-HA-FLAG

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-AU1

Plasmid

#162093

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to StrepTagII-HA-AU1

Use

Lentiviral

Tags

StrepTagII-HA-AU1

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-AU1

Plasmid

#162096

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to ProtC-HA-AU1

Use

Lentiviral

Tags

ProtC-HA-AU1

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-ProtC

Plasmid

#162079

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-StrepTagII-ProtC

Use

Lentiviral

Tags

VSVg-StrepTagII-ProtC

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-HA

Plasmid

#162083

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-ProtC-HA

Use

Lentiviral

Tags

VSVg-ProtC-HA

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLBM0010

Plasmid

#172816

Purpose

Level 2 module containing HSP21-GFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-GFP]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_Puro

Plasmid

#167895

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-TAF15 c1222t No Stop

Plasmid

#84900

Purpose

DONOR vector for Gateway cloning of TAF15 c1222t No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

TAF15 (TAF15 Human)

Use

Gateway donor

Tags

Expression

Mutation

c1222t No Stop

Promoter

none

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only
pOL2339

Plasmid

#37839

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Ub-V

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

nhx-2

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pOL2340

Plasmid

#37841

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Ub-M

Use

Tags

mCherry

Expression

Worm

Mutation

R77M

Promoter

nhx-2

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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AbVec_IGLC

Plasmid

#183703

Purpose

Encode human Ig lambda chain constant region. For cloning variable region and mAb expression.

Depositor

David Nemazee

Insert

IGLC2 (IGLC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAF0034 Ef1a-Kp03-2a-EGFP

Plasmid

#193461

Purpose

Expression of Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2D1

Plasmid

#15782

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)

Use

Tags

6x His, T7 tag, and Xpress tag (enterokinase clea…

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pBB542

Plasmid

#27395

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

dnaK, dnaJ, groESL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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GBX

Plasmid

#64123

Purpose

a modified episomal (EBNA1/OriP) vector expressing human eGFP and BCL-xL genes

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

GFP-2A-BCL2L1 (BCL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rDA3m

Plasmid

#208702

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3m

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEGIP

Plasmid

#26777

Purpose

Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

EF1a-GFP-IRES-Puro (gfp )

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pGFP Cortactin

Plasmid

#50728

Purpose

mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Insert

Cortactin (CTTN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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SIRT3 Flag

Plasmid

#13814

Purpose

Mammalian expression of human SIRT3 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT3 (SIRT3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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