163,517 results

1. pHH0103_YES1-1/1

   Plasmid

   #91439

   Purpose

   Protein expression and purification of human SH3 domain construct YES1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   YES1-1/1 (YES1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL1 sortase

   Plasmid

   #106288

   Purpose

   Expresses sortase in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   sortase A pentamutant
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXW117Ptet2-gfp

   Plasmid

   #160818

   Purpose

   aTc-responsive single-layered circuit

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

   Insert

   PJ23117-30tetR-t-Ptet2-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23117, Ptet2

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJH2930

   Plasmid

   #175283

   Purpose

   Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a of DAF-16

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   DAF-16a (daf-16 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Pges-1

   Available Since

   Dec. 3, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. CL20_mEGFP_EWSR1_ERG

   Plasmid

   #205819

   Purpose

   Express mEGFP-tagged fusion protein, EWSR1_ERG from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   EWSR1_ERG (EWSR1, ERG Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

   Plasmid

   #220735

   Purpose

   AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-TPNOX

   Plasmid

   #87853

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   TPNOX
   

   Use

   Tags

   C-terminal FLAG tag with a linker

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N3-FLuc-K0-Rluc_AH

   Plasmid

   #148846

   Purpose

   Mammalian Expression of K0-Rluc

   Depositor

   Elisa Izaurralde

   Insert

   K0-Rluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ACOT4A

   Plasmid

   #42449

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ACOT4A (ACOT4 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-Beta1

    Plasmid

    #140987

    Purpose

    Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GBeta1 (GNB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal human rhinovirus (HRV) 3C …

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-N2-COL1A1

    Plasmid

    #66602

    Purpose

    Expresses procollagen 1 alpha 1 as a GFP fusion

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

    Insert

    COL1A1 (COL1A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Puro-iNgn3

    Plasmid

    #75337

    Purpose

    doxycycline inducible Ngn3 over-expression

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

    Insert

    neurogenin 3 (Neurog3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. All-in-one lenti-ABE8e-NG

    Plasmid

    #200447

    Purpose

    Lentivirus vector for base editing

    Depositor

    Benjamin Cravatt

    Article

    Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-cerebody

    Plasmid

    #236738

    Purpose

    Protein overexpression of cerebody

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    cerebody
    

    Use

    Tags

    His, HA, FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_hSyn_NES-Kinprola_PKA-mEGFP_WRPE-SV40

    Plasmid

    #233366

    Purpose

    hSyn1 driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_PKA-mEGFP
    

    Use

    AAV

    Tags

    NES and mEGFP

    Expression

    Mutation

    Promoter

    hSyn1

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-HaloAktKAR2.2-T2A-EGFP

    Plasmid

    #216494

    Purpose

    Expression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAktKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

    Plasmid

    #216491

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1
    

    Use

    Tags

    NES, T2A-EGFP-CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6.3/TO/V5-DEST-mScarlet

    Plasmid

    #215536

    Purpose

    Lentiviral transfer vector to express the mScarlet fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    mScarlet
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. 8061-E19

    Plasmid

    #234256

    Purpose

    Gateway ORF Entry clone of human BMPR1B with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    BMPR1B (BMPR1B Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. 8061-E43

    Plasmid

    #234280

    Purpose

    Gateway ORF Entry clone of mouse Bmpr1b with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    Bmpr1b (Bmpr1b Mouse)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge