165,343 results

1. myc-hULK1

   Plasmid

   #31961

   Depositor

   Do-Hyung Kim

   Article

   Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

   Insert

   ULK1 (ULK1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Sept. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-8:TOMM20-mEGFP

   Plasmid

   #87423

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAG1159 TOM20-ECFP-FIREmate

   Plasmid

   #196693

   Purpose

   Expresses TOM20-ECFP-FIREmate in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   TOM20-ECFP-FIREmate
   

   Tags

   FIREmate

   Expression

   Mammalian

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJ609-dCas9-KR-puro

   Plasmid

   #158478

   Purpose

   Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells

   Depositor

   Brendan Price

   Article

   House et al PLoS One. 2020 Dec 17;15(12):e0237759. d

   Insert

   dCas9-KR
   

   Use

   CRISPR

   Tags

   KillerRed

   Expression

   Mammalian

   Mutation

   Cas9m4 (D10A+D839A+H840A+N863A)

   Promoter

   CMV

   Available Since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-MTOR-V2006I

   Plasmid

   #69012

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   V2006I

   Promoter

   CMV

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet-15b-microID

   Plasmid

   #172881

   Purpose

   expresses microID in E. coli

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   microID
   

   Tags

   myc

   Expression

   Bacterial

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   T7

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLminP_Luc2P_RE22

   Plasmid

   #90364

   Purpose

   STAT6 - Stat6 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   STAT6

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOlyA(WT)

   Plasmid

   #132391

   Purpose

   Ostreolysin A for sensing sphingomyelin/cholesterol complexes

   Depositor

   Arun Radhakrishnan

   Article

   Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

   Insert

   Ostreolysin A wild-type
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: C62S, C94S, S151C

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-Tet-OsTIR1(F74G)-V5

   Plasmid

   #158664

   Purpose

   Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   Tet-OsTIR1(F74G) AAVS1
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   F74G

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcsII-pb5-flag

    Plasmid

    #86769

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    proteasome beta subunit 5 (PSMB5 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. myc EDC4 pCMVSport6

    Plasmid

    #86855

    Purpose

    mammalian expression of EDC4

    Depositor

    Sarah Slavoff

    Article

    D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

    Insert

    EDC4 (EDC4 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSFFV_sfCherry2(11)_TagBFP

    Plasmid

    #82606

    Purpose

    Expresses sfCherry2(11) and TagBFP fusion in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed Gly12 to Ala in sfCherry11+

    Promoter

    SFFV

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK7-HA-S6K1-F5A

    Plasmid

    #8986

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F5A

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

14. mEmerald-ZO1-C-14

    Plasmid

    #54316

    Purpose

    Localization: Tight Junctions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    ZO1 (TJP1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pOlyA(E69A)

    Plasmid

    #132392

    Purpose

    Mutant version of OlyA that senses sphingomyelin without or with cholesterol

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A point mutant (E69A)
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, E69A, C94S, S151C

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA

    Plasmid

    #218529

    Purpose

    sgRNA targeting human EIF2AK1/HRI

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK1 (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3-EF1a-NICD1

    Plasmid

    #46047

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Promoter

    EF1a

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmRuby3-24xGCN4

    Plasmid

    #132413

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    mRuby3
    

    Expression

    Mammalian

    Mutation

    no

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTwist-SFFV-NFE2L18ND-Puro

    Plasmid

    #181918

    Purpose

    Lentiviral plasmid that directs the expression of mutant NFE2L1/Nrf1 where 8 putative N-glycosites are mutated from asparagine to aspartic acid in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    NFE2L1 (NFE2L1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    8 putative N-glycoyslation sites converted from a…

    Promoter

    SFFV

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-FLEX-GTB

    Plasmid

    #26197

    Depositor

    Edward Callaway

    Article

    Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

    Insert

    Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Mutation

    TVA, rabies glycoprotein are mammalian codon opti…

    Available Since

    Nov. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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