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SpyLigase

Plasmid

#51722

Purpose

Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

Depositor

Mark Howarth

Publication

Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

Insert

SpyLigase

Tags

His6 Tag

Expression

Bacterial

Promoter

T7

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-SrtAdelta59

Plasmid

#51138

Purpose

expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

Depositor

Hidde Ploegh

Publication

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.aureus SrtA delta 59

Tags

6x His

Expression

Bacterial

Promoter

T7

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pCMV3flag8HOIL-1L

Plasmid

#50016

Purpose

Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Publication

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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PCMV-intron myc Rab1aWT

Plasmid

#46776

Purpose

expression of WT Rab1a in mammalian cells

Depositor

Terry Hébert

Publication

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab1a (RAB1A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-Neo-Bam E2F3a

Plasmid

#37970

Depositor

Jacqueline Lees

Publication

Lees et al Mol Cell Biol. 1993 Dec;13(12):7813-25.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E2F3a (E2F3 Human)

Expression

Mammalian

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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LeGO-G2

Plasmid

#25917

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, eGFP

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pLenti-PGK-NRAS(G12V)

Plasmid

#35632

Depositor

Daniel Haber

Publication

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

N-Ras(G12V) (NRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pZW12 (CK2beta)

Plasmid

#27088

Depositor

David Litchfield

Publication

Turowec et al Methods Enzymol. 2010;484:471-93.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CK2beta (CSNK2B Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGEX-GST-AGO2

Plasmid

#24317

Depositor

Carl Novina

Publication

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

human AGO2 (AGO2 Human)

Tags

GST

Expression

Bacterial

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2

Plasmid

#28243

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

Depositor

Chandra Tucker

Publication

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEnt L1L3 EF1a-tTA-2

Plasmid

#26803

Depositor

Edward Hsiao

Publication

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Elongation Factor 1 alpha promoter and tTA transgene

Use

Gateway Cloning

Expression

Mammalian

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMV306hsp+Lux

Plasmid

#26159

Depositor

Brian Robertson, Siouxsie Wiles

Publication

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGL2B -1538/+64

Plasmid

#24942

Depositor

Stephen Smale

Publication

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEX-GFP-hLC3WT

Plasmid

#24987

Depositor

Isei Tanida

Publication

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

a wild type human MAP1-LC3B fused with EGFP (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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MigR1-AE

Plasmid

#12431

Depositor

Dong-Er Zhang

Publication

Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

Insert

AML1-ETO fusion (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

June 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3xFLAG-CMV10-hAtg13

Plasmid

#22872

Depositor

Noboru Mizushima

Publication

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

FLAG

Expression

Mammalian

Available since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pRR-PR-5Z

Plasmid

#23057

Depositor

Charles Miller

Publication

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

PR (PGR Human)

Expression

Yeast

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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WT-eNOS-RFP

Plasmid

#22497

Depositor

William Sessa

Publication

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WT eNOS

Tags

RFP

Expression

Mammalian

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Flag-HA-USP3

Plasmid

#22582

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP3 (USP3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMLM36 (aka pBR-UV5-GP-FD2)

Plasmid

#21871

Depositor

Keith Joung

Publication

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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