163,760 results

1. pCMV-PE6f

   Plasmid

   #207856

   Purpose

   Mammalian expression of PE6f prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6f
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

   Promoter

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-tadDE-PcrA M6-UGI

   Plasmid

   #221236

   Purpose

   Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   tadDE-PcrA M6-UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmal_c5x_RNAse_Inhib

   Plasmid

   #153314

   Purpose

   Bacterial expression of an RNase inhibitor

   Depositor

   Drew Endy, Philippa Marrack

   Insert

   RNAse Inhibitor
   

   Use

   Tags

   Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pECFP_C1_NSD2 FL

   Plasmid

   #221382

   Purpose

   Expresses NSD2 in mammalian cells

   Depositor

   Albert Jeltsch

   Article

   Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

   Insert

   Histone-lysine N-methyltransferase NSD2 (NSD2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L237R mutation in ECFP (Please see depositor comm…

   Promoter

   CMV

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCas9n(D10A)-Blast

   Plasmid

   #63593

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, nickase mutant

   Promoter

   EFS-NS

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3/td5StayGold(c4)=GianCreg

   Plasmid

   #212022

   Purpose

   For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GianCreg (GOLGB1 Human)
   

   Use

   Tags

   td5StayGold(c4)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-MBP-TEV

   Plasmid

   #69929

   Purpose

   bacterial expression of MBP-TEV n-terminal fusions

   Depositor

   Zita Balklava, Thomas Wassmer

   Article

   Currinn et al Cell Mol Life Sci. 2015 Jul 28.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   MBP and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZ M2rtTA_CAGG TetON-Sox10

   Plasmid

   #115240

   Purpose

   RMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Insert

   SOX10 (SOX10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. LRG2.1-BFP

   Plasmid

   #120577

   Purpose

   Lentiviral plasmid to co-express a guide RNA and EBFP.

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.2_miRFP670

    Plasmid

    #113726

    Purpose

    Expresses the fluorescent protein miRFP670 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    miRFP670
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. G6PD/pRK5

    Plasmid

    #41521

    Depositor

    Xiaolu Yang

    Article

    Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

    Insert

    glucose-6-phosphate dehydrogenase (G6PD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. FKBP-C-TEV 219

    Plasmid

    #100096

    Purpose

    SNIPer plasmid, C terminal TEV fused to FKBP

    Depositor

    Jim Wells

    Article

    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

    Insert

    C-TEV
    

    Use

    Retroviral

    Tags

    FKBP and Myc

    Expression

    Mammalian

    Mutation

    C terminal domain

    Promoter

    CMV

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MiniCoopR mitfa:EGFP

    Plasmid

    #118850

    Purpose

    Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    mitfa: EGFP (mitfa Synthetic, Zebrafish)
    

    Use

    Zebrafish expression, tol6

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH47751

    Plasmid

    #48002

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. EF1a-dCasRx-RBM25

    Plasmid

    #221001

    Purpose

    Transient expression of dCasRx-RBM25. EF1a promoter.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK1334

    Plasmid

    #127965

    Purpose

    sgRNA expression vector compatible with CROP-Seq and imaging screens

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

    Plasmid

    #188778

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV 3xFLAG-GABARAP WT

    Plasmid

    #123097

    Purpose

    Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212829

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Use

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG, TetOn

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR_TetO_CD19-P2A-mCherry

    Plasmid

    #133803

    Purpose

    repressible expression of CD19 and mCherry in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2018 Jul 13;361(6398):156-162.

    Insert

    putative ECD of H. sapiens CD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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