176,159 results
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Plasmid#75284PurposeExpresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cellsDepositorInsertPOLR2A (POLR2A Human)
TagsEYFPExpressionMammalianMutationN792D alpha-amanitin–resistantPromoterCMVAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA Flag Lamp2b-HA
Plasmid#71292PurposeFlag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.DepositorInsertLamp2b (LAMP2 Human)
TagsFlag, HA, and Lamp2 signal peptideExpressionMammalianPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_TNF-SBP-EGFP
Plasmid#65278Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorInsertTNF (TNF Human)
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSR58.6
Plasmid#63176PurposeExpresses CcaR constitutively and sfGFP under the PcpcG2-172 promoterDepositorInsertsCcaR
sfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBtet-Hyg
Plasmid#60508PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBMN-mCherry-Parkin
Plasmid#59419Purposeto make mCherry-Parkin stable cell line with retrovirusDepositorAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOTTC407 - pAAV EF1a eGFP
Plasmid#60058PurposeAn AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.DepositorInsertenhanced Green Fluorescent Protein
UseAAVExpressionMammalianPromoterEF1aAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p201N Cas9
Plasmid#59175PurposeCas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
nptII
UseCRISPRPromoter2x35S and StUbi-3PAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiPGK Puro DEST PKAKTRClover
Plasmid#59153PurposeLentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)DepositorAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-ARP2-N-14
Plasmid#54980PurposeLocalization: Actin Related Protein, Excitation: 587, Emission: 610DepositorAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DRH313: FCK-CheRiff-eGFP
Plasmid#51693PurposeChannelrhodopsin with improved sensitivity and minimal red light photocurrentDepositorInsertCheRiff-eGFP
UseLentiviralTagseGFPExpressionMammalianPromoterCaMKIIaAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFRT-TODestFLAGHAhFXR1
Plasmid#48694PurposeExpresses hFXR1DepositorInsertFlagHA FXR1 (isoform b) (FXR1 Human)
TagsFLAG and HAExpressionMammalianPromoterCMV, 2x TetAvailable sinceJune 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50463PurposeGq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoterDepositorInserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTALYM3B15
Plasmid#47878PurposeTALE-mClover against mouse major satellite sequenceDepositorInsertTALE-mClover
UseTALENTagsmCloverExpressionMammalianPromoterCAGAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickExpressionBacterialMutationR364S mutation in MKPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSPTg.T2FpAXK (#52)
Plasmid#35962DepositorInsertsTie2 promoter
SV40 pA
Tie2 enhancer
Available sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-Hb9-RFP
Plasmid#37081DepositorInsertHb9 RFP
UseLentiviralPromoterHb9Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-PGK-NRAS(G12V)
Plasmid#35632DepositorPublicationInsertN-Ras(G12V) (NRAS Human)
UseLentiviralTagsHAExpressionMammalianMutationGlycine 12 to ValinePromoterPGKAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ352 - MCS(ttTi4348, I)
Plasmid#34868DepositorInsertttTi4348 targeting
UseWorm targetingAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMXs-OLIG2
Plasmid#32933DepositorAvailable sinceNov. 30, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by