161,273 results

1. pRPF185

   Plasmid

   #106367

   Purpose

   C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

   Depositor

   Robert Fagan, Neil Fairweather

   Article

   Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

   Insert

   Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   codon optimised for C. difficile

   Promoter

   Ptet

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. ND4.2-Right DdCBE-G1397-C- T1413I-GFP

   Plasmid

   #179686

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T1413I

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-20nmEML-mRFP

   Plasmid

   #231181

   Purpose

   Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 20 nm from a lentiviral vector

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   20nmEML-mRFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. IF-GFP-ATRX

   Plasmid

   #45444

   Purpose

   ATRX expression vector

   Depositor

   Michael Dyer

   Article

   Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

   Insert

   ATRX (ATRX Mouse, Human)
   

   Use

   Tags

   GFP and HA

   Expression

   Bacterial and Mammalian

   Mutation

   isoform 2, missing codon E124

   Promoter

   PCMV

   Available since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-Neo-PTPRT (WT)

   Plasmid

   #16630

   Depositor

   Bert Vogelstein

   Article

   Wang et al Science. 2004 May 21. 304(5674):1164-6.

   Insert

   PTPRT (PTPRT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pNC-Cam1304-RNAi

   Plasmid

   #193399

   Purpose

   Nimble cloning-compatible vector for plant RNAi

   Depositor

   Pu Yan

   Article

   Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_PER1_WT

   Plasmid

   #82152

   Purpose

   Gateway Donor vector containing PER1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PER1 (PER1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXD054 KIKO CD19 CAR AAV vector

   Plasmid

   #192157

   Purpose

   CD19 CAR AAV vector

   Depositor

   Sidi Chen

   Article

   Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

   Insert

   anti-CD19 CAR (CD19 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-syn-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234438

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. SIRT2 Flag

    Plasmid

    #13813

    Purpose

    Mammalian expression of human SIRT2 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Transcript variant 2

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRCT

    Plasmid

    #60621

    Purpose

    Plasmid encoding iCas9, tracrRNA and crRNAs

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.GFAP.Cre.WPRE.hGH

    Plasmid

    #105550

    Purpose

    AAV expression of Cre from GFAP promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB and AAV5

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PSMC2_GFP_Cterm

    Plasmid

    #221516

    Purpose

    Fluorescently tagged Psmc2-GFP

    Depositor

    Alessandro Ori

    Insert

    PSMC2 (PSMC2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. SGEP

    Plasmid

    #111170

    Purpose

    miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    SFFV

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-BE-zCas9

    Plasmid

    #101739

    Purpose

    expression of an optimized Cas9 for base-editing in zebrafish

    Depositor

    Shuo Lin

    Article

    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

    Insert

    rAPOBEC1-XTEN-zCas9-UGI-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV

    Available since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQP-iRIS

    Plasmid

    #102584

    Purpose

    Regulation of protein localization (near-infrared-blue-light-inducible shuttle)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

    Insert

    BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-opto-mMLKL

    Plasmid

    #208784

    Purpose

    Inducibly expresses mouse MLKL fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    mCherry mMLKL Cry2 (Mlkl Mouse)
    

    Use

    Lentiviral

    Tags

    Cry2 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiGuideFE-Puro

    Plasmid

    #170069

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    Cas9 guide RNA scaffold with the F+E scaffold modification
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA6 asyn E46K

    Plasmid

    #105730

    Purpose

    Mammalian expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein E46K (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E46K

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCMV_FLUAV_HA

    Plasmid

    #207273

    Purpose

    Mammalian expression of Influenza A virus HA protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    FLUAV_HA (HA Influenza A virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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