163,486 results

1. pcDNA3.1-hSTING (S366A)

   Plasmid

   #214155

   Purpose

   Transient expression of STING

   Depositor

   Nan Yan

   Article

   Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

   Insert

   STING (S366A) (STING1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S366A

   Promoter

   CMV

   Available Since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-Tet-On shYap1-5

   Plasmid

   #193671

   Purpose

   Tet inducible knockdown of Yap1

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO-GAlphai3-RLuc8

   Plasmid

   #140975

   Purpose

   Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphai3-RLuc8 (GNAI3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLG010 (DRT type 2)

   Plasmid

   #157888

   Purpose

   Expresses the DRT type 2 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DRT type 2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1-Luc2-cG-BSD

   Plasmid

   #124982

   Purpose

   Express Luc2, copGFP

   Depositor

   Kazuhiro Oka

   Insert

   Luc2, copGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1-alpha

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-ChRmine-mScarlet-WPRE

   Plasmid

   #130994

   Purpose

   AAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAM1619

   Plasmid

   #40244

   Depositor

   Susan Golden

   Article

   Mackey et al Methods Mol Biol. 2007;362:115-29.

   Insert

   PpsbAI::luxCDE
   

   Use

   Cyanobacteria cloning vector

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-iCreV

   Plasmid

   #140135

   Purpose

   Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Has Service

   AAV PHP.eB and AAV1

   Insert

   iCreV
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO-GAlphaoA-RLuc8

   Plasmid

   #140976

   Purpose

   Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaoA-RLuc8 (GNAO1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

    Plasmid

    #61629

    Purpose

    R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

    Depositor

    Dolf Weijers

    Article

    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

    Inserts

    mDII
    ntdTomato
    DII
    n3xVenus
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-N-Puro-mStayGold-LMNB1

    Plasmid

    #227329

    Purpose

    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1+(rInsp)-Ins-GLuc

    Plasmid

    #89928

    Purpose

    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

    Depositor

    Melanie Cobb

    Article

    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

    Insert

    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DNA sequence for Gaussia luciferase was humanized…

    Promoter

    rat Insulin II promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 10279-M28-663

    Plasmid

    #225662

    Purpose

    Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCopGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mCMVp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-MbPylRS(DiZPK)

    Plasmid

    #91706

    Purpose

    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

    Depositor

    P. Chen

    Article

    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

    Inserts

    pylS
    pylT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

    Promoter

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF0589

    Plasmid

    #142653

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-CDK4

    Plasmid

    #116724

    Purpose

    Lentiviral expression of CDK4

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJRH-1364 LV EF1a-CD4 IRES-EGFP

    Plasmid

    #201920

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD4

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SDHC-pHLuorin-sems

    Plasmid

    #107767

    Purpose

    labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

    Depositor

    Karin Busch

    Article

    Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

    Insert

    Subunit C of SDH fused to pHluorin (SDHC Human)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AiP13777: pAAV-AiE0682h-minBG-iCre(R297T)-BGHpA (Alias: CN3777)

    Plasmid

    #214847

    Purpose

    Expression of iCre(R297T) in striatal Sst neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    R297T

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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