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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,258 results

Showing: 5221 - 5240 of 159258 results

  2. pUC57KAN-40XALFA

    Plasmid
    #233118
    Purpose
    40X ALFA tag multimer
    Depositor
    Steve Stowers
    Insert
    ALFA tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    233118-plasmid-map-sequence-id-467244

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  3. pUC57KAN-20XALFA

    Plasmid
    #233112
    Purpose
    20X ALFA tag multimer
    Depositor
    Steve Stowers
    Insert
    ALFA tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233112-plasmid-map-sequence-id-466717

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  4. pUC57KAN-20XHA

    Plasmid
    #233114
    Purpose
    20X HA tag multimer
    Depositor
    Steve Stowers
    Insert
    HA tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233114-plasmid-map-sequence-id-466707

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  5. pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234448
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234448-plasmid-map-sequence-id-465743

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  6. GB4080

    Plasmid
    #215249
    Purpose
    Module for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    SF-EGFP-p19-LUZ-H3H-HISPS-CPH
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    215249-plasmid-map-sequence-id-461241

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  10. pSBLego

    Plasmid
    #219967
    Purpose
    The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.
    Depositor
    Markus Seeger
    Article
    Ackle et al Sci Rep. 2025 Mar 19;15(1):9460. doi: 10
    Type
    Empty backbone
    Use
    Tags
    SLEHHHHHH and pelB signal sequence
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    219967-plasmid-map-sequence-id-435554

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  15. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-450823

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  16. GB4542

    Plasmid
    #215261
    Purpose
    Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    215261-plasmid-map-sequence-id-440598

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  17. AiP15050 - pAAV-AiE1426m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5050)

    Plasmid
    #224192
    Purpose
    AiE1426m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Sst_Chodl neurons
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    224192-plasmid-map-sequence-id-446785

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  18. GB4401

    Plasmid
    #215252
    Purpose
    Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    215252-plasmid-map-sequence-id-436586

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Showing: 5221 - 5240 of 159258 results