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pEN019-SpCas9-hiNLS-t-M1M4

Plasmid

#234456

Purpose

Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M1M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

Plasmid

#176271

Purpose

Expression of CasMINI-V3.1 in mammalian cells.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI-V3.1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_MFE1_hrGFP

Plasmid

#84617

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC22A6_STOP

Plasmid

#161131

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A6 (SLC22A6 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pInducer10b-EGFP-KRAS G12V

Plasmid

#164925

Purpose

Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells

Depositor

Ji Luo

Article

Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

Inserts

Enhanced green fluorescent protein
KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)

Use

Lentiviral

Mutation

Glycine 12 changed to valine (G->T 3156)

Promoter

Ubc Promoter and Ubc promoter

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pHIV-H2B-eGFP

Plasmid

#91776

Purpose

Expresses H2B protein tagged with eGFP

Depositor

Maria Pia Cosma

Article

Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

Insert

Human Histone H2B / eGFP (H2BC11 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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WT WWP1

Plasmid

#200085

Purpose

WWP1 in pQCXIN vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

WT WWP1 (WWP1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Ace-mNeon2-Kv2.1PR

Plasmid

#195528

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CaMKII

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Plasmid

#187334

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pNSEN-d2

Plasmid

#59763

Purpose

Expresses nuclear EGFP and Tomato in mammalian cells

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi

Inserts

d2-EGFP
Tomato

Tags

3X NLS and PEST

Expression

Mammalian

Promoter

CMV promoter and SV40 promoter

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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HaloTag-LaminA

Plasmid

#231894

Purpose

Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

LaminA (LMNA Human)

Tags

HaloTag

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP

Plasmid

#174876

Purpose

PiggyBac transposon vector for expression of RNAPII Ser2ph mintbody

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

RNAPII Ser2ph mintbody-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPBCAG-rtTAM2-IH

Plasmid

#140827

Purpose

Expresses rtTAM2-IRES-HygR in mammalian cells

Depositor

Hideyuki Okano

Article

Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

Inserts

rtTAM2
HygR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pgRNA-CKB

Plasmid

#73501

Purpose

gRNA expression vector (with mKate2)

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

mKate2-T2A-Bsd

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

Plasmid

#68375

Purpose

Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and TALEN; Zfn

Tags

3xFLAG_Twin_Strep

Expression

Mammalian

Promoter

human PGK1

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-taCasp3-TEVp

Plasmid

#45580

Depositor

Nirao Shah, Jim Wells

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Has Service

AAV1, AAV5, and AAV8

Inserts

Caspase 3 (CASP3 Synthetic, Human)
Tobacco Etch Virus protease

Use

AAV; Adeno associated viral vector

Mutation

Linker replaced with a TEV protease cleavage site

Promoter

EF1a

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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VPS13D^mScarlet

Plasmid

#174110

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Tags

mScarlet

Expression

Mammalian

Mutation

With an internal mScarlet tag in aminoacid positi…

Promoter

CMV

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Dlx-FLEX-hM4Di-mCherry

Plasmid

#209701

Purpose

Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

Depositor

Peer Wulff

Article

Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

Insert

hM4D(Gi)

Use

AAV

Tags

mCherry

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pICE-HA-NLS-I-PpoI

Plasmid

#46963

Purpose

For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

NLS-I-PpoI

Tags

HA

Expression

Mammalian

Promoter

CMV-TET

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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