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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 14440 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameERK1
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SpeciesM. musculus (mouse)
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Entrez GeneMapk3 (a.k.a. Erk, Erk-, Erk-1, Erk1, Ert2, Esr, Esrk1, Mnk1, Mtap, Mtap2k, Prk, Prkm3, p44, p44er, p44erk1, p44m, p44mapk)
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Tag
/ Fusion Protein
- T7 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Depositor Comments
T7 tag is MASMTGGQQMG.
Made by Sejeong Shin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-T7-ERK1 was a gift from John Blenis (Addgene plasmid # 14440 ; http://n2t.net/addgene:14440 ; RRID:Addgene_14440)