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pALPS puro miR30-IRF5

Plasmid

#117157

Purpose

Target site TATTTCCCTGTCTCCTTGGCC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF7

Plasmid

#117158

Purpose

Target site ATAAGGAAGCACTCGATGTCG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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SIRT3 Flag

Plasmid

#13814

Purpose

Mammalian expression of human SIRT3 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT3 (SIRT3 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA PSPC1

Plasmid

#101764

Purpose

Expresses PSPC1 in mammalian cells

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

paraspeckle component 1 (PSPC1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLyGo-Kp-1

Plasmid

#163143

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Neurofascin (extracellular) [A12/18R]

Plasmid

#177438

Purpose

Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE GFP

Plasmid

#10668

Purpose

Empty backbone for retroviral gene expression. Select recipient cells using GFP.

Depositor

William Hahn

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pDONR223_JAK2_WT

Plasmid

#81756

Purpose

Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JAK2 (JAK2 Human)

Use

Gateway entry vector

Mutation

V617F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP

Plasmid

#174876

Purpose

PiggyBac transposon vector for expression of RNAPII Ser2ph mintbody

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

RNAPII Ser2ph mintbody-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_ATP1.0

Plasmid

#167577

Purpose

Express the ATP sensor GRAB_ATP1.0 in neurons

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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spNW50

Plasmid

#173486

Purpose

Expresses spNW50 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW50

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-hPIKfyve

Plasmid

#121148

Purpose

Expresses GFP-tagged human PIKfyve in mammalian cells.

Depositor

Geert van den Bogaart

Article

Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

Insert

1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pgRNA-CKB

Plasmid

#73501

Purpose

gRNA expression vector (with mKate2)

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

mKate2-T2A-Bsd

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PX458-AAVS1

Plasmid

#113194

Purpose

Encodes gRNA for human AAVS1

Depositor

Adam Karpf

Insert

AAVS1 (PPP1R12C, AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-EGFP-WPRE

Plasmid

#51502

Purpose

Can be used to generate AAV virus that will express EGFP in the presence of Cre

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EGFP

Use

AAV

Promoter

CAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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2333_Cas9-CtIP-dnRNF168

Plasmid

#200254

Purpose

This plasmid expresses the Cas9-CtIP-dnRNF168 in mammalian cells

Depositor

Claudio Mussolino

Article

Carusillo et al Nucleic Acids Res. 2023 May 22;51(9):466

Insert

Cas9-CtIP-dnRNF168

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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LentiV_Cas9_puro

Plasmid

#108100

Purpose

Lentiviral expression plasmid of spCas9 with puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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CMV HaloTag-LgBiT Vector

Plasmid

#236917

Purpose

Mammalian expression vector encoding HaloTag(R)-LgBiT for use in HiBiT cell lines

Depositor

Promega Corporation

Has Service

DNA

Type

Empty backbone

Tags

HaloTag (R)-LgBiT

Expression

Mammalian

Mutation

None

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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