163,486 results

1. pLifeact-mScarlet3-H (mYongHong)

   Plasmid

   #239415

   Purpose

   Expresses Lifeact-mScarlet3-H (aka mYongHong) in mammalian cells

   Depositor

   Zhifei Fu

   Article

   Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

   Insert

   Lifeact-mScarlet3-H
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M163H in mScarlet3

   Promoter

   CMV

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

   Plasmid

   #214102

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti Lifeact-EGFP BlastR

   Plasmid

   #84383

   Purpose

   Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV EF1a DIO iChloC 2A dsRed

   Plasmid

   #70762

   Purpose

   An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)

   Depositor

   Thomas Oertner

   Article

   Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E83Q,E90R,E101S,D156N,T159C

   Promoter

   EF1a

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMTU-DO-G418-C4

   Plasmid

   #160342

   Purpose

   High-copy expression vector for K. marxianus, G418 selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet-TFIIF

   Plasmid

   #171082

   Purpose

   Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   RAP30 (GTF2F2 Human)
   RAP74 (GTF2F1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pColdI-GST-mGTSF1

   Plasmid

   #214671

   Purpose

   Bacterial expression of GST-tagged mouse GTSF1

   Depositor

   Phillip Zamore

   Article

   Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

   Insert

   GTSF1 (Gtsf1 Mouse)
   

   Use

   Tags

   6x His and GST

   Expression

   Bacterial

   Mutation

   Promoter

   CspA

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. RSV-Flag-Brd2

   Plasmid

   #86614

   Purpose

   Flag tagged mouse Brd2 expression construct for cell transfection

   Depositor

   Mario García-Domínguez

   Article

   Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

   Insert

   Brd2 (Brd2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   RSV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLSB-HYG

   Plasmid

   #166699

   Purpose

   Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   adh15 promoter and tRNA promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRXIP GFP-Stx18

    Plasmid

    #45918

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    syntaxin 18 (STX18 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pKmK.T4

    Plasmid

    #125056

    Purpose

    Storage plasmid for K.marxianus terminator; type 4 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    PDC1 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXL004-ishRNA-scramble

    Plasmid

    #36311

    Depositor

    Sean Palecek

    Article

    Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

    Insert

    shRNA of scramble
    

    Use

    Lentiviral and RNAi

    Tags

    EF1a-TetR-IRES-RFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-H1

    Available Since

    July 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac-M2

    Plasmid

    #135583

    Purpose

    High eukaryotic protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti CMV TRE3G Neo GFP-Progerin

    Plasmid

    #118710

    Purpose

    Lentiviral TET-ON inducible GFP-Progerin

    Depositor

    Tom Misteli

    Article

    Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

    Insert

    GFP-Progerin (LMNA Human)
    

    Use

    Lentiviral; Tet-on inducible

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TRE3G (TET-ON)

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFD3-dU6:3gRNA

    Plasmid

    #49410

    Purpose

    expression of gRNA under control of the Drosophila U6:3 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-3

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. BFP-KDEL

    Plasmid

    #49150

    Purpose

    visualization of the ER

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    BiP ER signal sequence (HSPA5 Human)
    

    Use

    Tags

    BFP and KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-RIIα-Dronpa

    Plasmid

    #182331

    Purpose

    PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    RIIalpha-Dronpa (PRKAR2A Human)
    

    Use

    Tags

    Full-length human PKA type 2alpha regulatory subu…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVS1-SAH2

    Plasmid

    #134746

    Purpose

    Replication helper

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    trfA-trfB-parDE
    

    Use

    Helper plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-Nopt

    Plasmid

    #231704

    Purpose

    Mammalian expression vector encoding a codon-optimized N gene from vesicular stomatitis virus, strain Indiana.

    Depositor

    Ron Geller

    Article

    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

    Insert

    VSV N gene (codon-optimized ) (VSIVgp1 vesicular stomatitis virus)
    

    Use

    Vsv expression

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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