pET21a(+)-Bst LF-6xHis
(Plasmid #159148)

• Purpose: expresses His-tagged large fragment of Bst polymerase
• Depositing Lab: Andrea Pauli
• Publication: Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. doi: 10.3389/fmolb.2022.801309. eCollection 2022.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: EMD Bioscience
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 7137
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: large fragment of Geobacillus stearothermophilus DNA polymerase I
• Alt name: Bst LF
• Species: Geobacillus stearothermophilus
• Insert Size (bp): 1776
• GenBank ID: AAB52611.1
• Tag / Fusion Protein:
  • His (C terminal on insert)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.06.23.166397v2 for bioRxiv preprint.