Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET28a_T7-ARG1
(Plasmid #106473)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 106473 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5374
  • Total vector size (bp) 12129
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARG1
  • Alt name
    Acoustic Reporter Gene 1
  • Alt name
    Hybrid Gas Vesicle gene cluster
  • Species
    Anabaena flos-aquae and Bacillus megaterium
  • Insert Size (bp)
    6755
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer T7_Forward
  • 3′ sequencing primer T7 terminator_Reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a_T7-ARG1 was a gift from Mikhail Shapiro (Addgene plasmid # 106473 ; http://n2t.net/addgene:106473 ; RRID:Addgene_106473)
  • For your References section:

    Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Bourdeau RW, Lee-Gosselin A, Lakshmanan A, Farhadi A, Kumar SR, Nety SP, Shapiro MG. Nature. 2018 Jan 3;553(7686):86-90. doi: 10.1038/nature25021. 10.1038/nature25021 PubMed 29300010