pTD103-ARG1
(Plasmid #106475)

• Purpose: Expresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimurium
• Depositing Lab: Mikhail Shapiro
• Publication: Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi: 10.1038/nature25021.

Backbone

• Vector backbone: pTD103
• Backbone manufacturer: Gift from Jeff Hasty
• Backbone size w/o insert (bp): 7293
• Total vector size (bp): 14048
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ARG1
• Alt name: Acoustic Reporter Gene 1
• Alt name: Hybrid Gas Vesicle gene cluster
• Species: Anabaena flos-aquae and Bacillus megaterium
• Insert Size (bp): 6755
• Promoter: pLux

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: custom primers
• 3′ sequencing primer: custom primers

Resource Information

• Supplemental Documents:
  • pTD103-ARG1.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that some discrepancies were found between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.