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pET28a_T5-ARG1
(Plasmid #106476)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 106476 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5189
  • Total vector size (bp) 11944
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin + 2% Glucose
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Turbo
  • Growth instructions
    Following transformation, rescue in SOC broth for 1 hour at 37C before transferring the rescued cells (entire volume) into overnight growth media (LB+ 50 ug/mL Kanamycin + 2% glucose) for growth at 37C. 2% glucose is optional, but it's recommend to prevent any leaky protein expression from the constitutive T5 promoter driving the ARG gene. Usually prepare a 40% stock solution of glucose and dilute it to the final concentration of 2%.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARG1
  • Alt name
    Acoustic Reporter Gene 1
  • Alt name
    Hybrid Gas Vesicle gene cluster
  • Species
    Anabaena flos-aquae and Bacillus megaterium
  • Insert Size (bp)
    6755
  • Promoter T5

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer custom primers
  • 3′ sequencing primer T7 terminator_Reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a_T5-ARG1 was a gift from Mikhail Shapiro (Addgene plasmid # 106476 ; http://n2t.net/addgene:106476 ; RRID:Addgene_106476)
  • For your References section:

    Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Bourdeau RW, Lee-Gosselin A, Lakshmanan A, Farhadi A, Kumar SR, Nety SP, Shapiro MG. Nature. 2018 Jan 3;553(7686):86-90. doi: 10.1038/nature25021. 10.1038/nature25021 PubMed 29300010