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PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 106474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5374
- Total vector size (bp) 11733
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameARG2
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Alt nameAcoustic Reporter Gene 2
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Alt nameHybrid Gas Vesicle gene cluster
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SpeciesAnabaena flos-aquae and Bacillus megaterium
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Insert Size (bp)6359
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7_Forward
- 3′ sequencing primer T7 terminator_Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a_T7-ARG2 was a gift from Mikhail Shapiro (Addgene plasmid # 106474 ; http://n2t.net/addgene:106474 ; RRID:Addgene_106474) -
For your References section:
Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Bourdeau RW, Lee-Gosselin A, Lakshmanan A, Farhadi A, Kumar SR, Nety SP, Shapiro MG. Nature. 2018 Jan 3;553(7686):86-90. doi: 10.1038/nature25021. 10.1038/nature25021 PubMed 29300010
Map uploaded by the depositor.
