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pST39-pNL29
(Plasmid #91696)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 91696 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pST39
  • Backbone manufacturer
    Tan Lab
  • Backbone size w/o insert (bp) 3119
  • Total vector size (bp) 8765
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mega GV gene cluster (from pNL29) in pST39 backbone
  • Alt name
    Mega GV gene cluster
  • Species
    Bacillus Megaterium
  • Insert Size (bp)
    5646
  • GenBank ID
    AF053765
  • Promoter T7
  • Tag / Fusion Protein
    • No tags

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Li, N. and Cannon, M.C. Gas Vesicle Genes Identified in Bacillus megaterium and Functional Expression in Escherichia coli. J Bacteriol. 1998 May; 180(9): 2450–2458.
  • Terms and Licenses
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pST39-pNL29 was a gift from Mikhail Shapiro (Addgene plasmid # 91696 ; http://n2t.net/addgene:91696 ; RRID:Addgene_91696)
  • For your References section:

    Preparation of biogenic gas vesicle nanostructures for use as contrast agents for ultrasound and MRI. Lakshmanan A, Lu GJ, Farhadi A, Nety SP, Kunth M, Lee-Gosselin A, Maresca D, Bourdeau RW, Yin M, Yan J, Witte C, Malounda D, Foster FS, Schroder L, Shapiro MG. Nat Protoc. 2017 Oct;12(10):2050-2080. doi: 10.1038/nprot.2017.081. Epub 2017 Sep 7. 10.1038/nprot.2017.081 PubMed 28880278