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WT C-His Ana GvpC in pET28a
(Plasmid #85732)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85732 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5206
  • Total vector size (bp) 5818
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gas vesicle protein C (his-tagged)
  • Alt name
    GvpC
  • Species
    Anabaena flos-aquae
  • Insert Size (bp)
    612
  • Mutation
    Added an extra glycine after the methionine at the N-terminus and have a 3 amino acid linker between GvpC and His-tag
  • Promoter T7
  • Tag / Fusion Protein
    • His-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 terminator reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WT C-His Ana GvpC in pET28a was a gift from Mikhail Shapiro (Addgene plasmid # 85732 ; http://n2t.net/addgene:85732 ; RRID:Addgene_85732)
  • For your References section:

    Molecular Engineering of Acoustic Protein Nanostructures. Lakshmanan A, Farhadi A, Nety SP, Lee-Gosselin A, Bourdeau RW, Maresca D, Shapiro MG. ACS Nano. 2016 Aug 23;10(8):7314-22. doi: 10.1021/acsnano.6b03364. Epub 2016 Jun 30. 10.1021/acsnano.6b03364 PubMed 27351374