163,606 results

1. pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

   Plasmid

   #162279

   Purpose

   Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

   Depositor

   Al George

   Article

   DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

   Insert

   SCN2A Variant 1, stabilized (SCN2A Human)
   

   Use

   Tags

   IRES-mScarlet

   Expression

   Mammalian

   Mutation

   IVS intron inserted after bp4551.

   Promoter

   CMV

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mammalian expression: Z-lock cofilin dark state mutant

   Plasmid

   #141136

   Purpose

   a control for optogenetic cofilin analog

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HMGCS2

   Plasmid

   #38984

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HMGCS2 (HMGCS2 Human)
   

   Use

   Tags

   His6-Thioredoxin-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. mCherry-LaminB1-10

   Plasmid

   #55069

   Purpose

   Localization: Nuclear Envelope, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEIGW Grx1-roGFP2

   Plasmid

   #64990

   Purpose

   Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Lentiviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-GFP-TurboID-NES_pLX304

   Plasmid

   #202019

   Purpose

   expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   Flag-GFP-TurboID-NES
   

   Use

   Lentiviral

   Tags

   Flag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-ExRai-AKAR2

   Plasmid

   #161753

   Purpose

   Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   ExRai-AKAR2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. 7TGC

   Plasmid

   #24304

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-eGFP // SV40-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   EGFP is GFP with S65T and F64L mutations

   Promoter

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmVenus(L68V)-mTurquoise2

   Plasmid

   #60493

   Purpose

   Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   SYFP2-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG FUCCI

    Plasmid

    #175266

    Purpose

    Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

    Depositor

    James Monaghan

    Article

    Duerr et al Development. 2022 Mar 15;149(6). pii: 27

    Insert

    FUCCI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA GFP10-ZIP

    Plasmid

    #182241

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP
    

    Use

    Tags

    split-GFP10 tripartite

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX209-neo-active WRN

    Plasmid

    #125788

    Purpose

    constitutive, ectopic expression of human active WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-active WRN (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEHlyA5

    Plasmid

    #168298

    Purpose

    Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

    Depositor

    Luis Ángel Fernández

    Article

    Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

    Insert

    N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
    

    Use

    Tags

    E-tag, HA-tag, His-tag, Prescission site, and VHH…

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-HA-RAD52-WT

    Plasmid

    #235676

    Purpose

    Expresses HA-tagged wild type human RAD52 in mammalian cells.

    Depositor

    Simon Powell

    Article

    Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

    Insert

    RAD52 (RAD52 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA ZIP-GFP11

    Plasmid

    #182242

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP11

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    ZIP-GFP11
    

    Use

    Tags

    split-GFP11 tripartite

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-PIK3C2B

    Plasmid

    #161989

    Purpose

    Mammalian expression vector containing PIK3C2B tagged with GFP

    Depositor

    Volker Haucke, David Bryant

    Article

    Marat et al Science. 2017 Jun 2;356(6341):968-972. d

    Insert

    PIK3C2B (PIK3C2B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    siRNA resistance to siRNA#2 with 4 silent mutatio…

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV enAsCas12f-HKRA-sgRNA_v7

    Plasmid

    #208357

    Purpose

    Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-HKRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I123H/D195K/D208R/V232A

    Promoter

    CMV

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDESTsplice

    Plasmid

    #32484

    Depositor

    Stefan Stamm

    Article

    Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLH01

    Plasmid

    #117261

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    PorfX-Lp_0640-42, sapK-sappR
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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