165,775 results

1. pTW2082

   Plasmid

   #236576

   Purpose

   ISYmu1 PDS3 gRNA12 TRV2 Architecture_B

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Insert

   ISYmu1 TnpB
   

   Expression

   Plant

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFL-EGFP-3xFlag-DEST

   Plasmid

   #134903

   Purpose

   Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Type

   Empty backbone

   Tags

   3x-Flag

   Expression

   Insect

   Promoter

   polyhedron

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHiUGE smFP-V5 donor (all-in-one) ORF0

   Plasmid

   #200386

   Purpose

   Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

   Insert

   spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. tetO-MITF

   Plasmid

   #170695

   Purpose

   doxycycline-inducible overexpression of MITF

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   MITF (MITF Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAXY0001

   Plasmid

   #179834

   Purpose

   binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

   Depositor

   Xiaohan Yang

   Article

   Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

   Insert

   eYGFPuv
   

   Use

   Binary vector for plant transformation

   Expression

   Plant

   Promoter

   35S

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. tdTomato-Integrin-Beta1-N-18

   Plasmid

   #58105

   Purpose

   Localization: Integrin/Focal Adhesions, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Insert

   Integrin-Beta1 (ITGB1 Human)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

7. Gg 3kb Green opsin GFP

   Plasmid

   #72919

   Purpose

   green opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving GFP

   Depositor

   Joseph Corbo

   Article

   Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

   Insert

   chicken green opsin promoter
   

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEVOL_PylRS(AF)-tRNA

   Plasmid

   #223511

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Inserts

   Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y306A; Y384F

   Promoter

   araBAD, rrnB, and glnS and proK

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCMV_JEV_prME

   Plasmid

   #207283

   Purpose

   Mammalian expression of Japanese encephalitis virus PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   JEV_PrME (POLY Japanese encephalitis virus)
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDestTol2pACryGFP

    Plasmid

    #64022

    Purpose

    Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

    Depositor

    Joachim Berger, Peter Currie

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    GFP
    

    Use

    Gateway

    Promoter

    alpha-Acrystallin promoter

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CAG-GFP

    Plasmid

    #28014

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Insert

    eGFP
    

    Use

    AAV; Adeno associate viral

    Available Since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-RET

    Plasmid

    #116787

    Purpose

    Lentiviral expression of RET

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    RET (RET Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pS548∙CsR

    Plasmid

    #197859

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Tc resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PEM7

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGTR

    Plasmid

    #63143

    Purpose

    PCR template to construct polycistronic tRNA-gRNA

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    gRNA-tRNA fusion
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. TR-Csy4-R115A/R119A

    Plasmid

    #80696

    Purpose

    Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    Csy4-R115A/R119A
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Arg115 and Arg119 both mutated to Ala

    Promoter

    CBA

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-nSpCas9-APOBEC3A

    Plasmid

    #209040

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    APOBEC3A-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZW6 (CK2alpha)

    Plasmid

    #27086

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha (CSNK2A1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. AiP15072 - pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA (Alias: CN5072)

    Plasmid

    #230402

    Purpose

    AiE0680m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHiUGE smFP-V5 donor (all-in-one) ORF1

    Plasmid

    #200387

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-U6-sgRNA-CMV-puro

    Plasmid

    #169450

    Purpose

    backbone for sgRNA expression

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    sgRNA
    

    Use

    Lentiviral

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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