163,570 results

1. pCMV-HA-UTX

   Plasmid

   #24168

   Purpose

   Mammalian expression of human UTX with HA tag

   Depositor

   Kristian Helin

   Article

   Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

   Insert

   UTX (KDM6A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRISPR-StAR4GN (Lenti)

   Plasmid

   #222693

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PGK without BsmBI cutsite

   Promoter

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-Skp2

   Plasmid

   #89142

   Purpose

   mammalian expression of human Skp2 with N-terminal FLAG tag

   Depositor

   Elizabeth Wilson

   Article

   Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

   Insert

   Skp2 (SKP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-dCasRx-ALKBH5-PURO

   Plasmid

   #175582

   Purpose

   Expresses dCasRx-ALKBH5 in mammalian cell

   Depositor

   Qi Xie

   Article

   Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

   Insert

   dCasRx-ALKBH5
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   RfxCas13d R239A/H244A/R858A/H863A

   Promoter

   pMSCV

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG LOC550643 pFCPGW

   Plasmid

   #86853

   Purpose

   retroviral expression of NoBody

   Depositor

   Sarah Slavoff

   Article

   D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

   Insert

   LOC550643 (NBDY )
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-EGFP-C1-HsPatL1_I

   Plasmid

   #146547

   Purpose

   Mammalian Expression of HsPatL1

   Depositor

   Elisa Izaurralde

   Article

   Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

   Insert

   HsPatL1 (PATL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGM4723:TpCC_Urease

   Plasmid

   #85982

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Inserts

   Cas9
   Urease-targeting gRNA 1
   Urease-targeting gRNA 2
   

   Use

   CRISPR; Diatom (t. pseudonana)

   Tags

   NLS and YFP

   Expression

   Mutation

   Promoter

   FCP and U6

   Available Since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

   Plasmid

   #179116

   Purpose

   Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   Cargo EGFP with AttP Bxb1 site for in-frame fusion
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1570

   Plasmid

   #144105

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF680 (ZNF680 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-GfaABC1D-MCS--4x6T-WPRE

    Plasmid

    #196417

    Purpose

    Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0101

    Plasmid

    #141515

    Purpose

    Lentiviral vector for overexpressing the FOXC2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXC2 (FOXC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSDest-HA-TMPRSS2

    Plasmid

    #154963

    Purpose

    TMPRSS2 with N-terminal HA

    Depositor

    Utpal Pajvani

    Article

    Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

    Insert

    HA-TMPRSS2 (TMPRSS2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pSAB35

    Plasmid

    #175787

    Purpose

    Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag

    Depositor

    John Rubinstein

    Article

    Abbas et al Science. 2020 Mar 13;367(6483):1240-1246

    Insert

    SidK (1-278)
    

    Use

    Tags

    3xFLAG and 6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. R777-E089 Hs.FGFR4

    Plasmid

    #70373

    Purpose

    Gateway ORF clone of human FGFR4 [NM_002011.4] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    FGFR4 (FGFR4 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH6HTC_IGF2BP2a-Halo

    Plasmid

    #175309

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IGF2BP2 (IGF2BP2 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458M-53BP1-DN1S

    Plasmid

    #131045

    Purpose

    Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

    Depositor

    Punam Malik

    Article

    Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

    Insert

    SpCas9-53BP1-DN1S (TP53BP1 Human)
    

    Use

    CRISPR

    Tags

    GFP and SpCas9

    Expression

    Mammalian

    Mutation

    Only expressing residues 1231-1644 of complete 53…

    Promoter

    CBH

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCaRNA1&2

    Plasmid

    #86087

    Purpose

    Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    RNA1, RNA2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-eCas9-GFP-U6-gRNA

    Plasmid

    #79145

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

    Depositor

    Jizhong Zou

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAR3-Lux

    Plasmid

    #24643

    Depositor

    Jeff Wrana

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pCaRNA3

    Plasmid

    #86088

    Purpose

    Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    RNA3
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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