159,333 results
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Plasmid#202083PurposeParent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-inDepositorInsertNT PuroR-T2A-mNG Minicircle Parent
UseCRISPRTagsmNeonGreenExpressionMutationPromoterNoneAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mLychee
Plasmid#219400PurposeConstitutive expression of mLychee in E. coliDepositorInsertmLychee
UseTagsExpressionBacterialMutationPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP
Plasmid#90008PurposeSplit-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx3
Plasmid#116855PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationamino acids 169-329 (tandem trimer)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM90
Plasmid#133647PurposeIntegration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).DepositorInsertpTPI1-SS-mCherry-HDEL
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ai139 (TIT2L-GFP-ICL-TPT) targeting vector
Plasmid#114426PurposeTarget a Cre-dependent GFP and a tdTomato-P2A-tTA2 expression cassette into the mouse TIGRE locusDepositorInsertGFP, tdTomato, tTA2,
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterTRE2, CAGAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSII-EF/mt-(n1)StayGold
Plasmid#185823PurposeA soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.DepositorInsertmt-(n1)StayGold
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-SB
Plasmid#177936PurposeExpresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.DepositorTypeEmpty backboneUseCRISPR; TransposonTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-TNFa-RUSH
Plasmid#166901PurposeRUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-HaloDepositorInsertHaloTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL2680
Plasmid#85581PurposeReplicating base vector for constructing cpf1/CRISPR editing plasmidsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-Inp54p
Plasmid#96812Purposecilia-membrane-targeted inositol polyphosphate 5-phosphataseDepositorInsert5HT6-YFP-Inp54p
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pS-cr:GFP
Plasmid#196295Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSsDepositorInsertFull length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
UseCRISPRTagsSpeI-DR-BsaI-DR-SpeIExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)
Plasmid#125146PurposeAn adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoterDepositorInsertHA-tagged hM4D(Gi) (CHRM4 Human)
UseAAV and Cre/LoxTagsHAExpressionMammalianMutationPromoterSYN1Available sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-MCS-ASC-GFP
Plasmid#73957PurposeExpress human ASC with a C-terminal EGFP tag in mammalian cellsDepositorInsertPYD and CARD domain containing, PYCARD (PYCARD Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
DT480
Plasmid#80413PurposeExpresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#161928PurposeDonor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and Citrine activation reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpEFAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6f
Plasmid#182548PurposeExpresses GCaMP6f on the cytosolic side of the ER (P-450site)DepositorInsertER localized P450
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-ORF-V2
Plasmid#73638PurposeGateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).DepositorTypeEmpty backboneUseTagsVenus fragment 2 (V2)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
UseTagsExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FGFR2-N549K
Plasmid#116380PurposeLentiviral expression of FGFR2 N549KDepositorInsertFGFR2 (FGFR2 Human)
UseLentiviralTagsExpressionMutationN549KPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only