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pLX401-INK4A

Plasmid

#121919

Purpose

Doxycycline-inducible expression of p16-INK4A product of CDKN2A

Depositor

William Hahn

Article

Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

Insert

p16-INK4A (CDKN2A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE Tet ON

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSCM001

Plasmid

#124605

Purpose

Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

Depositor

David Summers

Article

Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

Insert

mCherry-pHluorin

Tags

mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

Expression

Bacterial

Promoter

pDAB

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1354

Plasmid

#119275

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET-15b-TEV-C9R

Plasmid

#118754

Purpose

TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

Depositor

Yutaka Kuroda

Article

Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

Insert

Tobacco Etch Virus protease (NEWENTRY Synthetic)

Tags

C9R and His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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PKC delta WT

Plasmid

#16386

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC delta isoform 2 (Prkcd Mouse)

Tags

HA

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1

Plasmid

#187120

Purpose

mammalian cell expression of FLAG-HUWE1

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRGD-SmR

Plasmid

#74109

Purpose

Plasmid containing streptomycin resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

aadA

Use

Bacterial vector based on pmb1 replicon

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163

Plasmid

#127192

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDG02583

Plasmid

#104129

Purpose

Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

NEDD8-specific protease 1 (NEDP1)

Tags

14x Histidine tag, Maltose binding protein, and S…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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PB-sgRNA(MS2)

Plasmid

#102560

Purpose

Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204341

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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mNeonGreen-KDEL

Plasmid

#226568

Purpose

Encodes mNeonGreen with KDEL ER lumenal target sequence

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

KDEL (ER lumenal target sequence)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-IRF1

Plasmid

#232641

Purpose

transient expresses IRF1 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

IRF1 (IRF1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pKD239-1g-2

Plasmid

#90959

Purpose

Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Tetrathionate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23100, Bba_J23109, and PttrB185-269

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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spNW100

Plasmid

#173488

Purpose

Expresses spNW100 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW100

Tags

Histag

Expression

Bacterial

Mutation

P1049L- please see depositor comments

Promoter

T7

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9

Plasmid

#61433

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Expression

Plant

Promoter

PcUBI4-2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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AiP12689 - pAAV-AiE0682h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2689)

Plasmid

#191727

Purpose

AI plasmid ALIAS: AiP12689. Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICa:ER-MT (High Affinity)

Plasmid

#247995

Purpose

Expresses the SPLICa reporter for ER-Mitochondria contacts and Calcium transients at the contact

Depositor

Tito Calì

Article

Yamaguchi et al Anal Chem. 2025 Sep 16;97(36):19848-1986

Insert

SPLICa ER-MT (h)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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SuperClomeleon

Plasmid

#203724

Purpose

An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride

Depositor

George Augustine

Article

Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.

Insert

Cerulean linked topaz fluorescent protein

Expression

Bacterial and Mammalian

Mutation

Cerulean, modified Topaz Fluorescent Protein with…

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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