165,688 results

1. pEVOL-pAzF[TAG]

   Plasmid

   #164579

   Purpose

   Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

   Depositor

   Ryan Mehl

   Article

   Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

   Inserts

   Mj pCNFRS[TAG] (aaRS1)
   Mj pCNFRS[TAG] (aaRS2)
   Mj tRNA[TAG]
   

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   araBAD, glnS, and proK

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SIN40C.SFFV.Luciferase.IRES.GFP

   Plasmid

   #169308

   Purpose

   Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Promoter

   SFFV

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7-VEE-mCherry

   Plasmid

   #193355

   Purpose

   Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PcDNA3.1-Flag

   Plasmid

   #210342

   Purpose

   Empty Vector for molecular cloning

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   Flag tag
   

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-E-Syt2

   Plasmid

   #66831

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt2 (ESYT2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-NLRC5

   Plasmid

   #37521

   Depositor

   Thomas Kufer

   Article

   Neerincx et al J Biol Chem. 2010 Aug 20;285(34):26223-3

   Insert

   NLRC5 (NLRC5 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-hTimeless (pc4729)

   Plasmid

   #216861

   Purpose

   Mammalian expression vector encoding GFP tagged human Timeless

   Depositor

   Cristina Cardoso

   Article

   Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

   Insert

   Timeless (TIMELESS Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-HDAC4

   Plasmid

   #45636

   Depositor

   Tso-Pang Yao

   Article

   Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

   Insert

   HDAC4 (HDAC4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKM-U6-reRNA-acceptor-2xBbsI

   Plasmid

   #205633

   Purpose

   Cloning and U6-drive expression of ISDra2 TnpB reRNAs

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

    Plasmid

    #172436

    Purpose

    Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    Cre
    mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SMN2E7-Fluc-Nluc-reporter

    Plasmid

    #221005

    Purpose

    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    Fluc-SMN2E7-STOP-Nluc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. EB3N-VVDfast-mCl3-iLID

    Plasmid

    #190165

    Purpose

    Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    EB3N-VVDfast-mClover3-iLID
    

    Tags

    -VVDfast-mClover3-iLID

    Expression

    Mammalian

    Mutation

    1-189 aa from EB3

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Cre-IRES2-GFP

    Plasmid

    #26646

    Purpose

    Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

    Depositor

    Anjen Chenn

    Article

    Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    GFP and IRES2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCX459

    Plasmid

    #229214

    Purpose

    CRISPR array plasmid for MAP1B (Spacers 1-24)

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    CRISPR array plasmid for MAP1B (Spacers 1-24)
    

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid

    #216152

    Purpose

    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with cryptic exon and C-terminal FLAG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-Cas9-gRNA-TagBFP2

    Plasmid

    #124774

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p101_CRISPRai_PiggyBac

    Plasmid

    #213777

    Purpose

    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    

    Use

    CRISPR

    Promoter

    EF1a (EF-1-alpha intron a) and Tet_On_TRE

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-PD-1 (Y223F, Y248F)-mGFP

    Plasmid

    #180790

    Purpose

    Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    PD-1 (Y223F, Y248F) (PDCD1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Mutation

    PD-1 (Y223F, Y248F)

    Promoter

    SFFV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-TOM20_Halo_T2A_EGFP

    Plasmid

    #135443

    Purpose

    Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    TOM20-HaloTag7 and EGFP
    

    Tags

    TOM20

    Expression

    Mammalian

    Promoter

    CMV/TetO

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCMV_DENV1_PrME

    Plasmid

    #207263

    Purpose

    Mammalian expression of Dengue virus 1 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV1_PrME (POLY Dengue virus 1)
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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