179,844 results
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Plasmid#118706Purposereporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferaseDepositorInsertHRE (HIF-responsive promoter (3xHRE)-luc
UseLentiviralAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
plenti-CAG-gate-FLAG-IRES-GFP
Plasmid#107398PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseGateway Cloning and LentiviralTagsFLAGExpressionMammalianAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
miniTurbo-V5_pRS415
Plasmid#107168Purposeexpresses V5-tagged miniTurbo in the yeast cytosolDepositorInsertminiTurbo (BirA mutant)
ExpressionYeastMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCW57.1 N-term GFP tTA
Plasmid#107551PurposeAll-in-one dox-repressible expression of cDNA with optional N-term GFP tag.DepositorTypeEmpty backboneUseLentiviralTagsGFP (optional)ExpressionMammalianAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-PKA-SPARK
Plasmid#106920PurposeExpress PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKA-SPARK
ExpressionMammalianAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCN-EF2132tet
Plasmid#107191PurposeExpresses EF2132 recombinase for performing recombineering in S. aureusDepositorInsertsEF2132
tetR
ExpressionBacterialPromoterp23Available sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CMVc-Cas9
Plasmid#106431PurposeExpresses SpCas9 from the CMVc promoter in AAV backboneDepositorInsertCas9
UseAAV and CRISPRTagsHA-NLS and NLSExpressionMammalianPromoterCMVcAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-ZNF598-TEV-3xFLAG
Plasmid#105690PurposeExpresses C terminally 3xFLAG tagged ZNF598 proteinDepositorAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCSC-ISL1-T2A-LHX3
Plasmid#90215PurposeTo convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.DepositorAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lck-mTurquoise2
Plasmid#98822PurposeIn vivo visualization of the plasma membrane (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLck
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCW57-RFP-P2A-MCS (Neo)
Plasmid#89182PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…ExpressionMammalianPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ223 (dAsCpf1-SRDX)
Plasmid#86209PurposeDeactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmidDepositorInsertdAsCpf1
UseGateway compatible cpf1 vectorsTagsNLS and SRDX repressor and NLSExpressionPlantMutationD908AAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT3-Neo-EF1a-GFP
Plasmid#69134PurposeSleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promotersDepositorTypeEmpty backboneUseSleeping beauty transposonExpressionMammalianPromoterU3MSCVAvailable sinceJan. 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PR_GEF (2XPDZ-mCherry-Larg (DH)
Plasmid#80407PurposeRecruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)
TagsmCherryExpressionMammalianMutationDH domain only (aa 766-997)PromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-ER GFP
Plasmid#80069PurposeTo express and target GFP into the endoplasmic reticulum using lentivirusDepositorInsertER GFP (SEC61B Human)
UseLentiviralTagsGFP derived from Aequorea coerulescens on the NH2…ExpressionMammalianPromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC18R6K-mini-Tn7T-Gm
Plasmid#65022PurposeGmR mini-Tn7 vector, R6K repliconDepositorPublicationTypeEmpty backboneAvailable sinceAug. 17, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
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pU6b:sgRNA#3
Plasmid#64247Purposeexpresses sgRNA under U6b promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by