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PCE(cm24Cre)

Plasmid

#242425

Purpose

for chromosome editing

Depositor

Caixia Gao

Article

Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

Insert

PE, cm24Cre

Use

CRISPR

Expression

Plant

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCEP9 MLH1

Plasmid

#16458

Depositor

Bert Vogelstein

Article

Papadopoulos et al Science. 1994 Mar 18. 263(5153):1625-9.

Insert

MLH1 (MLH1 Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human PP2A Aα full length

Plasmid

#132634

Purpose

expresses human PP2A Aα full length in E. coli

Depositor

Wenqing Xu

Article

Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

Insert

PP2A Aα (PPP2R1A Human)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH

Plasmid

#180149

Purpose

This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.

Depositor

Xin Chen

Article

Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

Type

Empty backbone

Expression

Mammalian

Promoter

EF1α

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

Plasmid

#60228

Purpose

Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA

Expression

Mammalian

Promoter

CBh and U6

Available Since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pLOX-Ttag-iresTK

Plasmid

#12246

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

SV40 Large T, HSV1-TK (SV40gp6 Simian virus)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

Plasmid

#51509

Purpose

Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV1

Insert

tdTomato-T2A-SypEGFP

Use

AAV

Promoter

phSyn1

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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rEstus

Plasmid

#209767

Purpose

To express a bright and sensitive genetically encoded voltage indicator in mammalian cells.

Depositor

Stefan Heinemann

Article

Ruhl et al Adv Sci (Weinh). 2024 May;11(20):e230793

Insert

rEstus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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1XDR4

Plasmid

#32614

Depositor

Connie Cepko

Article

Billings et al PLoS One. 2010 . 5(10):e13739.

Insert

1XDR4

Expression

Mammalian

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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Halo-53BP1 HRD

Plasmid

#207087

Purpose

Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-lck-GCaMP6f

Plasmid

#52924

Purpose

Fluorescent reporter for calcium signaling

Depositor

Baljit Khakh

Has Service

AAV5

Insert

lck-GCaMP6f

Use

AAV

Tags

6xHis

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113341

Purpose

Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Expression

Mammalian

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG_WT MDA5

Plasmid

#216798

Purpose

Expresses wild type (wt) melanoma differentiation-associated protein 5

Depositor

Sun Hur

Insert

melanoma differentiation-associated protein 5 (IFIH1 Human)

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_PB_Poetur

Plasmid

#248198

Purpose

Poetur transposase expressing plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

Poetur hyPB

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

Plasmid

#188767

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLeAPS-GFP-blast

Plasmid

#182230

Purpose

Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

GFP-P2A-Blasticidin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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NYESO beta/alpha into TRAC HDRT Source (pTR 169)

Plasmid

#112021

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta/alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Available Since

Sept. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE56

Plasmid

#247034

Purpose

Glucocorticoid Receptor pathway reporter; lentiviral expression of NR3C1 (GR) DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NR3C1 (GR)

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b-6h-GST-TEVp

Plasmid

#172887

Purpose

Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Insert

TEV protease

Tags

GST solubility tag and HHHHHH

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAV1K-T5-gfp

Plasmid

#26702

Depositor

Ichiro Matsumura

Article

Bryksin et al PLoS One. 2010 . 5(10):e13244.

Insert

T5lacOlacOeGFP biobrick

Expression

Bacterial

Available Since

Nov. 15, 2010

Availability

Academic Institutions and Nonprofits only

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