165,688 results

1. Str-KDEL_ST-SBP-EGFP

   Plasmid

   #65264

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-f:PGC1

   Plasmid

   #1026

   Depositor

   Bruce Spiegelman

   Article

   Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

   Insert

   PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)/Luc2=tdT

   Plasmid

   #32904

   Purpose

   Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

   Depositor

   Christopher Contag

   Article

   Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

   Inserts

   Firefly Luciferase
   tandem Tomato Red Fluorescent Protein
   

   Tags

   tdTomato red fluorescent protein

   Expression

   Mammalian

   Promoter

   CMV-IE and n/a

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOpen-P45

   Plasmid

   #165570

   Purpose

   Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   P45
   

   Use

   Synthetic Biology

   Available Since

   Aug. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. human p53-(1-393)

   Plasmid

   #24859

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Full length.

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. Olig001

   Plasmid

   #170716

   Purpose

   Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

   Depositor

   Thomas McCown

   Article

   Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

   Insert

   Olig001 capsid gene / rep 2
   

   Use

   AAV

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRUPATH Triple Ga11

   Plasmid

   #196059

   Purpose

   Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha11-RLuc8 (GNA11 Human)
   GGamma13-GFP2 (GNG13 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-PML IV/pRK5

   Plasmid

   #59742

   Purpose

   Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

   Depositor

   Xiaolu Yang

   Article

   Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

   Insert

   PML isoform IV (PML Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPlatTET-gRNA2

   Plasmid

   #82559

   Purpose

   All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

   Depositor

   Izuho Hatada

   Article

   Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

   Insert

   dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
   

   Expression

   Mammalian

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_AGIA-AirID

    Plasmid

    #182210

    Purpose

    Express AirID in mammalian cells.

    Depositor

    Shogo Nakano, Tatsuya Sawasaki, Sohei Ito, Akira Nozawa

    Article

    Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

    Insert

    AGIA-AirID
    

    Tags

    AGIA-tag

    Expression

    Mammalian

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVSV eGFP dG

    Plasmid

    #31842

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    VSV genome
    

    Expression

    Bacterial

    Available Since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMH-Halo tag

    Plasmid

    #154144

    Purpose

    Gateway compatible mammalian expression plasmid for halo tag fusion proteins

    Depositor

    Michael Huen

    Type

    Empty backbone

    Tags

    Halo tag

    Expression

    Mammalian

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MCP-MSN

    Plasmid

    #210704

    Purpose

    This plasmid express EF1a promoter driven MCP-MSN transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. FUGW-APPSwe-T2A-mCherry

    Plasmid

    #190804

    Purpose

    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Article

    Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Mutation

    Swedish (KM670/671NL)

    Promoter

    Ubi C

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Rosa26 mT/mG

    Plasmid

    #17787

    Depositor

    Liqun Luo

    Article

    Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

    Insert

    Rosa26 mT/mG
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    membrane tag

    Expression

    Mammalian

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-Cas9-2A-GFP-sgRNA

    Plasmid

    #124889

    Purpose

    Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

    Depositor

    Harvey Lodish

    Article

    Li et al Haematologica. 2016 Jun;101(6):e216-9. d

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    2A-GFP

    Promoter

    MSCV

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet-LahT150

    Plasmid

    #166785

    Purpose

    Cleavage of double glycine motif in peptides to remove the leader sequence

    Depositor

    Wilfred van der Donk

    Article

    Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

    Insert

    LahT150
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. 2C::tdTomato Reporter

    Plasmid

    #40281

    Depositor

    Samuel Pfaff

    Article

    Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

    Insert

    2C Promoter (2-cell stage promoter)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Available Since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCTRE-CD4

    Plasmid

    #114010

    Purpose

    Dox-inducible Cas9 expression with a CD4 selection marker

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA

    Plasmid

    #154867

    Purpose

    Flp-dependent hM4D(Gi) DREADD-mCherry

    Depositor

    Ulrik Gether

    Article

    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

    Has Service

    AAV Retrograde and AAV8

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Human Synapsin-1

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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