165,688 results

1. pICH41766

   Plasmid

   #48018

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pR-mAmetrine

   Plasmid

   #228598

   Purpose

   Mammalian expression of mAmetrine, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mAmetrine
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AbVec1.1-IGLC2-XhoI

   Plasmid

   #99575

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hCMV IE1 gene promoter

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMBP-MS2

   Plasmid

   #65104

   Purpose

   can be used to isolate RNA binding complexes

   Depositor

   Josep Vilardell

   Article

   Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

   Insert

   MS2
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   double mutation (V75Q and A81G) that prevents oli…

   Promoter

   tac

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

   Plasmid

   #126049

   Purpose

   For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

   Depositor

   François Bachand

   Article

   Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

   Insert

   TurboID
   

   Use

   Pcr-based c-terminal tagging

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. V5-GFP-APEX2-NES_pLX208

   Plasmid

   #202018

   Purpose

   expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-GFP-APEX2-NES
   

   Use

   Lentiviral

   Tags

   GFP and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pgk-ATG-frt

   Plasmid

   #20734

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   pgk-ATG-frt-SAdpA-tetO
   

   Use

   Mouse Targeting

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. 4xNFkB Luc

   Plasmid

   #111216

   Purpose

   reporter gene

   Depositor

   Johannes A. Schmid

   Insert

   firefly Luciferase
   

   Expression

   Mammalian

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9 (PX165)

   Plasmid

   #48137

   Purpose

   Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS_L_EBOV

    Plasmid

    #103052

    Purpose

    Encodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    L gene of Ebola virus (L Zaire ebolavirus, Mayinga)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid

    #215010

    Purpose

    Express a Mitophagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    pSu9 (Presequence of Fo-ATPase subunit 9)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-CMV-jGCaMP7f

    Plasmid

    #104483

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7f
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLIK-Hygro

    Plasmid

    #25737

    Purpose

    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. px330-mcherry

    Plasmid

    #98750

    Purpose

    Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

    Depositor

    Jinsong Li

    Article

    Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFD152

    Plasmid

    #125546

    Purpose

    derivative of pFD116 with an optimized RBS for E. coli

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Insert

    optimized RBS
    

    Use

    CRISPR

    Promoter

    anhydrotetracycline Ptet promoter

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRE3G-human cGAS-HA

    Plasmid

    #186842

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS with C-terminal HA tag (Adamts1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. LAMP1-RpHLuorin2

    Plasmid

    #171720

    Purpose

    Lysosomal expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
    

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPAmCherry1-N1

    Plasmid

    #31928

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    cmv

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cas9 TLS1

    Plasmid

    #196977

    Purpose

    Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9 with TLS1 tag
    

    Expression

    Plant

    Promoter

    pG10-90

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)Flag-His-ATM wt

    Plasmid

    #31985

    Purpose

    Mammalian expression of human ATM with Flag and His tags

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM wild-type (ATM Human)
    

    Tags

    Flag and HIS

    Expression

    Mammalian

    Mutation

    3' UTR of 400 nucl.

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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