Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

169,328 results

Showing: 5501 - 5520 of 169328 results

  2. TRUPATH Triple Ga13

    Plasmid
    #196062
    Purpose
    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196062-plasmid-map-sequence-id-385881

    Enlarge

  5. TFORF1500

    Plasmid
    #143960
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RBL2 (RBL2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143960-plasmid-map-sequence-id-404618

    Enlarge

  12. pCW57.1_SPTLC1_C133W

    Plasmid
    #181921
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W
    Depositor
    Christian Metallo
    Article
    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline i…
    Expression
    Mammalian
    Mutation
    cysteine at position 133 of the coding region of …
    Promoter
    tight TRE promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    181921-plasmid-map-sequence-id-361675

    Enlarge

  13. pANAP

    Plasmid
    #48696
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS
    Depositor
    Peter Schultz
    Article
    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540
    Inserts
    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV and human H1 promoter
    Available Since
    Nov. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    48696-plasmid-map-sequence-id-197837

    Enlarge

  18. pCDH-EF1-IRES eGFP

    Plasmid
    #128059
    Purpose
    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128059-plasmid-map-sequence-id-256675

    Enlarge

Showing: 5501 - 5520 of 169328 results