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TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

Plasmid

#216156

Purpose

Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with C-terminal FLAG tag

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX_311-Cas9

Plasmid

#96924

Purpose

for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

Depositor

John Doench, William Hahn, David Root

Article

Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

Insert

Cas9

Use

Lentiviral

Tags

3xFlag and V5

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_CK8e_Cas9_miniPA

Plasmid

#226141

Purpose

AAV construct for HITI insert with CK8e promoter and SpCas9

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Insert

Cas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CK8e

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-3xFLAG-His6-LARP1

Plasmid

#233626

Purpose

Expression of Flag- and His-tagged LARP1

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

LARP1 (LARP1 Human)

Tags

3FLAG-His6

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPROBE-NT

Plasmid

#37818

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotorless gfp reporter gene

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pLexM-SLC7A11_GFP

Plasmid

#197995

Purpose

Expresses SLC7A11 with an N-terminal FLAG tag and C-terminal TEV-GFP-His8 tag in mammalian cells

Depositor

Simon Newstead

Article

Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

Insert

SLC7A11 (SLC7A11 Human)

Tags

FLAG tag and TEV-GFP-His8

Expression

Mammalian

Promoter

CMV

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-SMARCA4

Plasmid

#65391

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SMARCA4 (SMARCA4 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shNS

Plasmid

#127696

Purpose

Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shNS

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pETDuet1_His-TEV-EGFP

Plasmid

#223723

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

EGFP, not monomeric (has A207)

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-AID-BubR1

Plasmid

#47330

Purpose

for plasmid integration using the Flp-In system. AID at N-terminal

Depositor

Don Cleveland

Article

Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

Insert

BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)

Tags

AID and GFP

Expression

Mammalian

Mutation

siRNA resistant

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pLIX403_Capture1_APOBEC_HA_P2A_mRuby

Plasmid

#183901

Purpose

Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

Capture1_APOBEC_HA_P2A_mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19

Plasmid

#78597

Purpose

Express flag-USP19 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 (USP19 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-KRAB-Puro

Plasmid

#99372

Purpose

3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-KRAB-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163031

Purpose

FLiCRE TF with fast kinetics for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-f-hLOV1-TEVcs-tTA

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBOB-VE-cadherin-GFP

Plasmid

#118732

Purpose

To express VE-cadherin tagged with GFP on C-terminus

Depositor

John Cooper

Article

Kim et al Mol Biol Cell. 2018 Jul 15;29(13):1693-1

Insert

Cadherin 5 (CD144) (CDH5 Human)

Use

Lentiviral

Tags

GFP

Mutation

I503T (Common SNP-see comments)

Promoter

CMV

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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NanoBRET Assay Vector, HaloTag-KRAS

Plasmid

#236871

Purpose

Express HaloTag(R)-KRAS in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

KRAS (KRAS Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET Assay Vector, NanoLuc-SOS1cat

Plasmid

#236848

Purpose

Express NanoLuc-SOS1cat in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

SOS1cat (SOS1 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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ColorfulCell

Plasmid

#62449

Purpose

MXS_chaining vector with 6 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(-30)GFP

Plasmid

#62936

Purpose

Expression of 6xHis-(-30)GFP in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Hygro DEST (w117-1)

Plasmid

#17454

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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