Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,606 results

Showing: 5561 - 5580 of 163606 results

  1. pMF230

    Plasmid
    #62546
    Purpose
    Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.
    Depositor
    Michael Franklin
    Article
    Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.
    Insert
    GFP mut2
    Use
    Broad host range plasmid
    Tags
    Expression
    Mutation
    Promoter
    trc
    Available Since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    62546-plasmid-map-sequence-id-207090

    Enlarge

  2. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

    Enlarge

  3. dSpCas9-VPR

    Plasmid
    #205247
    Purpose
    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSpCas9-VPR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    205247-plasmid-map-sequence-id-408440

    Enlarge

  7. pMCRW1

    Plasmid
    #122462
    Purpose
    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells
    Depositor
    Richard C. Willson
    Article
    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10
    Insert
    NPM-ALK fusion protein (ALK, NPM1 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available Since
    May 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    122462-plasmid-map-sequence-id-243544

    Enlarge

  8. pCS2+HyPer7-NLS

    Plasmid
    #136468
    Purpose
    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
    Depositor
    Vsevolod Belousov
    Article
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Insert
    HyPer7
    Use
    Tags
    Triple nuclear localization signal of SV40 (simia…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SP6
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    136468-plasmid-map-sequence-id-308715

    Enlarge

  9. pTA-Mob 2.0

    Plasmid
    #149662
    Purpose
    Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.
    Depositor
    Bogumil Karas
    Article
    Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:
    Type
    Empty backbone
    Use
    Synthetic Biology; Conjugation helper plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    149662-plasmid-map-sequence-id-379005

    Enlarge

  10. pVQ CMV NanoV1-2a-EGFP ferritin

    Plasmid
    #79649
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanillin 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79649-plasmid-map-sequence-id-175067

    Enlarge

  18. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

    Enlarge

Showing: 5561 - 5580 of 163606 results