165,764 results

1. pF155 pcDNA3.1(+)SOD1G93A

   Plasmid

   #26401

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Expression

   Mammalian

   Mutation

   G93A

   Available Since

   Nov. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-N-FLAG-HA-TBK1

   Plasmid

   #131791

   Purpose

   Lentiviral vector expressing WT TBK1

   Depositor

   Alicia Pickrell

   Article

   Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

   Insert

   TBK1 WT (TBK1 Human)
   

   Use

   Lentiviral

   Tags

   N-FLAG-HA

   Expression

   Mammalian

   Mutation

   WT full length

   Promoter

   CMV

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKL003

   Plasmid

   #98919

   Purpose

   Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

   Insert

   PROPS
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   BBa_J23118 - Strong Constitutive

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC4-RhE-FRB-Fis1

   Plasmid

   #68056

   Purpose

   express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

   Depositor

   Richard Youle

   Article

   Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

   Insert

   Fis1 (FIS1 Human)
   

   Tags

   FRB

   Expression

   Mammalian

   Mutation

   Fis1 tail (aa 93-152)

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. GW1-pHRed

   Plasmid

   #31473

   Purpose

   genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

   Depositor

   Gary Yellen

   Article

   Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

   Insert

   pHRed
   

   Expression

   Mammalian

   Available Since

   July 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBad-CA

   Plasmid

   #105665

   Purpose

   expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Tags

   6x his tag

   Expression

   Bacterial

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. LAMP1-RpHLuorin2

   Plasmid

   #171720

   Purpose

   Lysosomal expression of ratiometric pHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
   

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG PTEN

   Plasmid

   #78777

   Purpose

   To overexpress PTEN in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

   Insert

   PTEN (PTEN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

   Plasmid

   #207860

   Purpose

   AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
   

   Use

   AAV

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)

    Plasmid

    #203909

    Purpose

    Encodes wild type Gal4-VP64 transcription factor in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    Gal4-VP64-IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTriEx-NLyn-Venus-Zdk1

    Plasmid

    #81042

    Purpose

    mammalian expression of plasma-membrane anchored Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Tags

    6x His tag, FLAG, NLyn, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-Neo-mtkeima

    Plasmid

    #210338

    Purpose

    Overexpression of mtkeima

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mtkeima
    

    Expression

    Mammalian

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV2_HLF-ZP

    Plasmid

    #175035

    Purpose

    HLF targeting construct introducing an ires ZsG P2A Puro cassette downstream of the human HLF stop codon

    Depositor

    Guy Sauvageau

    Article

    Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

    Insert

    HLF ires ZsGreen P2A Puro
    

    Use

    AAV

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS0035

    Plasmid

    #131125

    Purpose

    Mammalian Target-AID expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1 and UGI

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV promoter

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a-Traptavidin

    Plasmid

    #24753

    Depositor

    Mark Howarth

    Article

    Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

    Insert

    Traptavidin
    

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    Streptavidin with 2 amino acid mutations: S52G an…

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHelper_NoMutL_V2_gentR

    Plasmid

    #205293

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a-KLF2-T2A-mCHERRY

    Plasmid

    #224295

    Purpose

    Expresses mouse KLF2 and mCherry under EF1a promoter

    Depositor

    Nikhil Joshi

    Article

    Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

    Insert

    KLF2 T2A mCherry (Klf2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHCMV_DENV2_PrME

    Plasmid

    #207264

    Purpose

    Mammalian expression of Dengue virus 2 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV2_PrME (POLY Dengue virus 2)
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-BsaI

    Plasmid

    #54340

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Type

    Empty backbone

    Use

    Entry vector

    Available Since

    March 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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