166,033 results

1. pET28a_His-TEV-EiCsm6

   Plasmid

   #172487

   Purpose

   Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

   Insert

   Enterococcus italicus Csm6 (codon optimized)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7 promoter and lac operator

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EGFP-C1-centrin-1

   Plasmid

   #73331

   Purpose

   Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag

   Depositor

   Manuel Thery

   Insert

   centrin-1 (CETN1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-GCN5

   Plasmid

   #65386

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   GCN5 (KAT2A Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223-LRPPRC

   Plasmid

   #23521

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   LRPPRC (LRPPRC Human)
   

   Use

   Gateway donor vector

   Mutation

   G246A; deleted E579

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

   Plasmid

   #174007

   Purpose

   Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

   Depositor

   Mark Histed

   Article

   LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

   Has Service

   AAV9

   Insert

   jGCaMP8s-P2A-ChrimsonR-ST
   

   Use

   AAV

   Tags

   6xHis and soma targeting motif

   Promoter

   hSyn

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. HaloTag-bActinCDS-bActinUTR-MS2V5

   Plasmid

   #102718

   Purpose

   Halo-Actin reporter for detection of both mRNA and protein

   Depositor

   Robert Singer

   Article

   Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   HaloTag-bActinCDS-bActin3'UTR-MS2V5
   

   Use

   Lentiviral

   Tags

   HaloTag and MS2V5

   Promoter

   UbC

   Available Since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-FGFR3

   Plasmid

   #116742

   Purpose

   Lentiviral expression of FGFR3

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   FGFR3 (FGFR3 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Split 'n coexpress CUL3-RBX1

   Plasmid

   #52293

   Purpose

   bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1

   Depositor

   Brenda Schulman

   Article

   Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

   Inserts

   Cul3 N-terminal domain (CUL3 Human)
   Cul3 C-terminal domain (CUL3 Human)
   RBX1 (Rbx1 Mouse)
   

   Tags

   GST, IRES, and TEV

   Expression

   Bacterial

   Mutation

   contains aa 1-309, contains aa 310-768, and conta…

   Promoter

   IRES and tac

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. lenti-sgRNA hygro

   Plasmid

   #104991

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. His6-SpyCatcher-mi3

    Plasmid

    #113043

    Purpose

    Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

    Insert

    His6-SpyCatcher-mi3
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-puro/Flag PD-L1 WT

    Plasmid

    #121446

    Purpose

    expression of huamn PD-L1 wildtype in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    wildtype

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYIC

    Plasmid

    #18673

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Type

    Empty backbone

    Tags

    3Myc-EYFP-HA-His6 and ECFP-HA-His6

    Expression

    Mammalian

    Available Since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-dual-CRISPR-U6-H1(pBP43)

    Plasmid

    #218930

    Purpose

    The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    human U6 and H1

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX-EF1a ANXA11-mEmerald

    Plasmid

    #164210

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-R2Tg_opt-3xFlag

    Plasmid

    #223251

    Purpose

    Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPLICSFAST-ER-PO-P2A

    Plasmid

    #214894

    Purpose

    Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-ER-PO-P2A
    

    Tags

    none

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOpen-phi29pol

    Plasmid

    #165573

    Purpose

    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    phi29 DNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pTP264

    Plasmid

    #149334

    Purpose

    Bacterial expression of Biotin ligase BirA

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Biotin ligase BirA
    

    Tags

    His14 and bdNEDD8

    Expression

    Bacterial

    Available Since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEos4b-TOMM20-N-10

    Plasmid

    #57518

    Purpose

    Localization: Mitochondrial Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson, Loren Looger

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mEos4b

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. mEGFP-PI35P2

    Plasmid

    #92419

    Purpose

    Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

    Insert

    MCOLN1 (Mcoln1 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Synthetic gene, codon optimized for human.

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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