161,813 results

1. pSIN-LexAOx4-Pmin-mCherry

   Plasmid

   #192633

   Purpose

   Lentiviral vector for inducible mCherry expression.

   Depositor

   Yingxiao Wang

   Article

   Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal promoter

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLNHA-C1-HseIF4AI_K

   Plasmid

   #146731

   Purpose

   Mammalian Expression of HseIF4AI

   Depositor

   Elisa Izaurralde

   Insert

   HseIF4AI (EIF4A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   a non silent mutation (A200V) and 3 silent mutati…

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPOU5F1-donor

   Plasmid

   #176367

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

   Plasmid

   #160628

   Purpose

   Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos, 35S, 35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)

   Plasmid

   #160566

   Purpose

   GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   Multiplexing Edit (E3-E4-En-1)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Septin 11 isoform II

   Plasmid

   #120318

   Purpose

   Septin 11 isoform II expression (mCherry-tag at the N-terminus)

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSP64T Activin betaB (DM#108)

   Plasmid

   #15045

   Depositor

   Douglas Melton

   Article

   Thomsen et al Cell. 1990 Nov 2. 63(3):485-93.

   Insert

   Activin Beta b (inhbb Frog)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

8. LentiGuide-Hygro

   Plasmid

   #139462

   Purpose

   Lentiviral vector with gRNA scaffold and hygromycin selectable marker

   Depositor

   Caroline Goujon

   Insert

   no sgRNA inserted; resistance gene: hygroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PBCAG-mRFP

   Plasmid

   #40996

   Depositor

   Joseph Loturco

   Article

   Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

   Insert

   mRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. reporter-gT1

    Plasmid

    #47320

    Purpose

    TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    minCMV-dTomato_pA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-H2B-iRFP720

    Plasmid

    #128961

    Purpose

    Lentiviral plasmid for expression of far red iRFP720 nuclear marker

    Depositor

    Carlos Carmona-Fontaine

    Insert

    Human histone H2B (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    iRFP720

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHIV-iRFP720-E2A-Luc

    Plasmid

    #104587

    Purpose

    Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

    Depositor

    Antonius Plagge

    Article

    Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

    Insert

    iRFP720-E2A-Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    bi-cistronic

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PLKO.1-Scrambled

    Plasmid

    #136035

    Purpose

    Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    None (Scrambled)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hsyn-GRAB_DA2m

    Plasmid

    #140553

    Purpose

    Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    The 2nd generation DA sensor GRAB_DA2m (medium affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LV-Sox10MCS5-GFP

    Plasmid

    #115783

    Purpose

    This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.

    Depositor

    Fraser Sim

    Article

    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

    Insert

    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    

    Use

    Lentiviral

    Tags

    cfos basal promoter conjugated MCS5 promoter fuse…

    Expression

    Mutation

    Promoter

    SOX10 Multiple Species Conserved enhancer element…

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHDM-Tat1b

    Plasmid

    #164442

    Purpose

    Encodes HIV-1 Tat accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Tat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-hPIASx beta

    Plasmid

    #15210

    Depositor

    Ke Shuai

    Article

    Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

    Insert

    PIASx beta (PIAS2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPROEX-TwStrp-FTO

    Plasmid

    #190265

    Purpose

    Expresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.

    Depositor

    Samie Jaffrey

    Insert

    FTO (FTO Human)
    

    Use

    Tags

    6xHis and Twin-Streptag

    Expression

    Bacterial

    Mutation

    Promoter

    Trc

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-MP2

    Plasmid

    #36097

    Purpose

    3rd generation lentiviral plasmid

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid

    #85452

    Purpose

    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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