163,607 results

1. pUC19-HRP-C

   Plasmid

   #40163

   Depositor

   Paul Ortiz de Montellano

   Article

   Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

   Insert

   Horseradish peroxidase
   

   Use

   Coli cloning vector

   Tags

   Six histidine

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_SFFv_4D5-High-CAR_RHL002

   Plasmid

   #164825

   Purpose

   Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Insert

   AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIBN(deltaNLS)-pmGFP

   Plasmid

   #26867

   Purpose

   Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   NLS sequences mutated

   Promoter

   CMV

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti Lifeact-mRuby2 BlastR

   Plasmid

   #84384

   Purpose

   Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-SLC16A1_STOP

   Plasmid

   #161100

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC16A1 (SLC16A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAdTrack-CMV

   Plasmid

   #16405

   Purpose

   Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Sac-Abeta(M1-42)

   Plasmid

   #71875

   Purpose

   Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-42) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSAB35

   Plasmid

   #175787

   Purpose

   Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag

   Depositor

   John Rubinstein

   Article

   Abbas et al Science. 2020 Mar 13;367(6483):1240-1246

   Insert

   SidK (1-278)
   

   Use

   Tags

   3xFLAG and 6xHis TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cav1-GFP

   Plasmid

   #14433

   Depositor

   Ari Helenius

   Article

   Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

   Insert

   Caveolin-1 (CAV1 Canis familalis)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEmerald-CLIP170-N-18

    Plasmid

    #54044

    Purpose

    Localization: Microtubule Binding , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CLIP170 (CLIP1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid

    #183409

    Purpose

    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4-NHK-MYC-His6

    Plasmid

    #185350

    Purpose

    Assessing of ubiquitination in cells

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    a1-antitrypsin Hong Kong Null mutant
    

    Use

    Tags

    Myc, 6xHis

    Expression

    Mammalian

    Mutation

    NHK mutant

    Promoter

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-No-HaloTag7-mGFP-LC3

    Plasmid

    #184901

    Purpose

    Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. GAPDH-CPG-TV

    Plasmid

    #70148

    Purpose

    a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
    

    Use

    Mouse Targeting and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

    Plasmid

    #209082

    Purpose

    NFIB and SOX9 mediated astrocye induction

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6MFV SLC10A1

    Plasmid

    #194130

    Purpose

    Expresses NTCP (SLC10A1) protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC10A1 (SLC10A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-CAG-hCas3

    Plasmid

    #134920

    Purpose

    Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas3 with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-HRE-dUnaG

    Plasmid

    #124372

    Purpose

    UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

    Depositor

    Roland Friedel

    Article

    Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

    Insert

    HRE-dUnaG
    

    Use

    Lentiviral

    Tags

    PEST-degron and Myc tag

    Expression

    Mutation

    Promoter

    HRE (HIF responsive element 5x + CMV minimal prom…

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCVpuro-TagRFP657

    Plasmid

    #96939

    Purpose

    Retroviral plasmid containing TagRFP657 for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Tag Red Fluorescent Protein 657
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

    Plasmid

    #101060

    Purpose

    AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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