166,034 results

1. pEVOL_PylRS(AF)-tRNA

   Plasmid

   #223511

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Inserts

   Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y306A; Y384F

   Promoter

   araBAD, rrnB, and glnS and proK

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TetO-FUW-NICD

   Plasmid

   #61540

   Purpose

   Expresses NOTCH-ICD in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   NOTCH-ICD (NOTCH1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   NOTCH intracellular domain (aa1761-2555); A1943S…

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ICUE3

   Plasmid

   #61622

   Purpose

   FRET-based biosensor for monitoring cAMP

   Depositor

   Jin Zhang

   Article

   DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

   Insert

   ICUE3 (RAPGEF3 Human)
   

   Tags

   ECFP and cpVenus[L194]

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-HIF1alpha P402A/P564A-pcDNA3

   Plasmid

   #18955

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 402 to Alanin…

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. HRE:GFP-pGK:Cre

   Plasmid

   #68552

   Purpose

   Lentivirus expressing hypoxia-responsive GFP reporter and Cre

   Depositor

   Tyler Jacks

   Inserts

   Green Fluorescent Protein
   Cre
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   HRE and pGK

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Voltron-ST

   Plasmid

   #119034

   Purpose

   Express soma-localized Voltron in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

   Insert

   Voltron-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPLICS Po-ER Long P2A

   Plasmid

   #164113

   Purpose

   Detect the long Peroxisomes-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP Po-ER Long P2A
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUS-mCh-sspB

   Plasmid

   #227377

   Purpose

   Condensate module, light-inducible oligomerization IDR

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   FUS IDR
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB CAG-sfGFP

   Plasmid

   #133568

   Purpose

   CAG sfGFP piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Expression

   Mammalian

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. scFLARE2

    Plasmid

    #158700

    Purpose

    Neuronal expression of scFLARE2 component

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    scFLARE2
    

    Use

    AAV

    Tags

    HA, Flag, VP16

    Expression

    Mammalian

    Mutation

    Truncated form of TEV protease (220-242 amino aci…

    Promoter

    Syn

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOpen-SapIR

    Plasmid

    #165537

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    SapI
    

    Use

    Synthetic Biology

    Available Since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

    Plasmid

    #218205

    Purpose

    Zebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

    Depositor

    Katarzyna Koltowska

    Article

    Panara et al Development. 2024 May 1;151(9):dev202525

    Insert

    -2.1prox1a
    

    Use

    Zebrafish expression

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIDTSmart-MbPylRS

    Plasmid

    #217361

    Purpose

    Expresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmid

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    M. barkeri pyrrolysyl-tRNA synthetase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBP-AaCas12b

    Plasmid

    #113433

    Purpose

    Expresses Expresses 10xHis-MBP fused to AaCas12b

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AaCas12b
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHLmMBP-11

    Plasmid

    #72349

    Purpose

    Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

    Expression

    Mammalian

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCmini-iCAP-AAV.MaCPNS2

    Plasmid

    #185137

    Purpose

    non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

    Insert

    Synthetic construct isolate AAV-MaCPNS2 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV5B-TGFbeta receptor II wt (N-term HA)

    Plasmid

    #24801

    Depositor

    Jeff Wrana

    Insert

    TGFbeta receptor II (TGFBR2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pRCA918 - TFORF Empty Vector Puro-P2A-BFP (pRCA892 backbone)

    Plasmid

    #238183

    Purpose

    Lentiviral empty vector

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVSVΔG-eGFP-linker

    Plasmid

    #231707

    Purpose

    Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

    Depositor

    Ron Geller

    Article

    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

    Insert

    VSV antigenome
    

    Use

    Vsv expression

    Expression

    Mammalian

    Mutation

    ΔG (VSV glycoprotein removed)

    Promoter

    T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-TrxRFP2

    Plasmid

    #172322

    Purpose

    an improved fluorescent biosensor for thioredoxin redox

    Depositor

    Huiwang Ai

    Article

    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

    Insert

    TrxRFP2, an improved version from TrxRFP1
    

    Tags

    His-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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