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pBMN YFP-DHFR(DD)

Plasmid

#29326

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

YFP-DHFR(DD)

Use

Retroviral

Tags

HA and YFP

Mutation

Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-n1-APP

Plasmid

#69924

Purpose

mammalian expression of human APP fused to EGFP

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

APP (APP Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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AmCyan-P2A-mCherry

Plasmid

#45350

Depositor

Ilpo Huhtaniemi

Article

Potorac et al J Endocrinol. 2016 Sep 21. pii: JOE-16-0

Insert

AmCyan

Tags

mCherry

Expression

Mammalian

Mutation

SV40 NLS

Promoter

CMV

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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AiP12315 - pAAV-AiE0464m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2315)

Plasmid

#223981

Purpose

AiE0464m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g6

Plasmid

#80789

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV p16 INK4A

Plasmid

#10916

Depositor

Bob Weinberg

Article

Medema et al Proc Natl Acad Sci U S A. 1995 Jul 3. 92

Insert

p16 INK4 (CDKN2A Human)

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pDUET-1-alpha-synuclein-mCerulean3-His6

Plasmid

#110061

Purpose

For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Insert

alpha-synuclein (SNCA Human)

Tags

6 x His and mCerulean3

Expression

Bacterial

Promoter

T7

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDDRFP-A1

Plasmid

#36289

Depositor

Robert Campbell

Article

Alford et al Chem Biol. 2012 Mar 23;19(3):353-60.

Insert

Dimerization dependent RFP A1

Tags

His tag

Expression

Bacterial

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)_No_FLAG_AAVS1_T2

Plasmid

#79888

Purpose

Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

Plasmid

#102422

Purpose

Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

Depositor

Kian Peng Koh

Article

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Insert

Tet1 catalytic domain mutant (Tet1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

H1620Y and D1622A mutations in Tet1 catalytic dom…

Promoter

TetO-CMV

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDDRFP-B1

Plasmid

#36290

Depositor

Robert Campbell

Article

Alford et al Chem Biol. 2012 Mar 23;19(3):353-60.

Insert

Dimerization dependent RFP-B1

Tags

His

Expression

Bacterial

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
MAC_C_MUSK

Plasmid

#187781

Purpose

MAC-tagged gene expression of human MUSK

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

MUSK (MUSK Human)

Expression

Mammalian

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SpG‐ABE8e(V106W)

Plasmid

#187069

Purpose

ABE8e(V106W)-SpG

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

SpG‐ABE8e(V106W)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCryptDel4.8

Plasmid

#141293

Purpose

Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Inserts

RelB
gRNA targetting pMUT2

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Native promoter from pMUT2 relB/relE operon and P…

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-NFAT luciferase

Plasmid

#17870

Purpose

3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

Depositor

Jerry Crabtree

Article

Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

Insert

3x NFAT binding sequence (IL2 )

Use

Luciferase

Tags

IL2 promoter and Luciferase

Expression

Mammalian

Promoter

IL-2 promoter

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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nls-transposase-nls-pA in pT3TS-Dest

Plasmid

#140873

Purpose

Vector for in vitro transcription of Tol2 nls-transposase-nls for zebrafish transgenesis using T3.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nls-transposase-nls (Tol2)

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET29-bdSUMO-Anti-ALFA nanobody

Plasmid

#212311

Purpose

Expression of anti-ALFA nanobody

Depositor

Arthur Laganowsky

Article

Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

Insert

anti-ALFA nanobody

Tags

bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGADT7-GW

Plasmid

#61702

Purpose

Gateway compatible Y2H vector - AD

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

Plasmid

#187320

Purpose

dCas9 expression vector with cloning site for C terminal fusions

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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