165,550 results

1. pcDNA3.1-HaloAktKAR2.2-T2A-EGFP

   Plasmid

   #216494

   Purpose

   Expression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAktKAR2.2
   

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA HA HP1 alpha

   Plasmid

   #24078

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 alpha (CBX5 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pFUW-tetO-NFKB1

   Plasmid

   #139822

   Purpose

   doxycycline-inducible expression of Human NFKB1 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   NFKB1 (NFKB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-mito-tagRFP

   Plasmid

   #185599

   Purpose

   Lentiviral construct for expression of tagRFP-T targeted to mitochondria

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   mito-tagRFP-T
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ultra-Exon1Q145-Myc-A

   Plasmid

   #110487

   Purpose

   Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)

   Depositor

   Baoji Xu

   Article

   Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

   Insert

   Homo sapiens huntingtin (HTT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Partial long 3'UTR wild type huntingtin exon…

   Promoter

   UbC

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cbh_v5 AAV-ABE C-terminal

   Plasmid

   #137178

   Purpose

   AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE C-terminal; U6-protospacer
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMTU-DO-URA

   Plasmid

   #160004

   Purpose

   Low-copy expression vector for K. marxianus, uracil selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   n/a

   Expression

   Yeast

   Promoter

   n/a

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. phCMV-GALV-MTR

   Plasmid

   #163612

   Purpose

   Retro and Lentiviral transduction of primary human germinal center B cells

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Insert

   GaLV MTR envelope
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. DsRed-rab11 WT

   Plasmid

   #12679

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB11A (Homo sapiens) (RAB11A Human)
   

   Tags

   DsRed and HA

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUSE-URA3-Sec7-msYFP2

    Plasmid

    #218969

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2

    Depositor

    Benjamin Glick

    Insert

    SEC7-msYFP2 (SEC7 Budding Yeast)
    

    Tags

    msYFP2

    Expression

    Yeast

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-EGFP-NLS

    Plasmid

    #165441

    Purpose

    Expresses nuclear EGFP from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    EGFP-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPbU6_hdhfr/yfcu_Cas9

    Plasmid

    #216423

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Expression

    Bacterial

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Talin1-mCherry

    Plasmid

    #212004

    Purpose

    Expression of mouse full-length (1-2541) talin1-mCherry in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

    Insert

    Mouse talin1 (Tln1 Mouse)
    

    Tags

    mCherry fluorescent protein

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.4_CEACAM6-His

    Plasmid

    #149337

    Purpose

    for eukaryotic expression and purification of CEACAM6-His6

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Tags

    IL-2 signal peptide and polyhistidine

    Expression

    Mammalian

    Mutation

    point mutation (GGA to GCA) to replace 'G-g…

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMVTO-SEAP-26Sfur-B2AD-tevs-KKMP

    Plasmid

    #179636

    Purpose

    TEVP-inducible single TM SEAP RELEASE construct

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    SEAP RELEASE
    

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-PGK-Venus-Akaluc (neo)

    Plasmid

    #124701

    Purpose

    Aka-Luciferase reporter

    Depositor

    Roland Friedel

    Article

    Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

    Insert

    Venus-Akaluciferase
    

    Use

    Lentiviral

    Promoter

    hPGK

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1α-mNeonGreen-Z1

    Plasmid

    #135613

    Purpose

    mNeonGreen monomeric fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.

    Depositor

    Jordan Green

    Insert

    mNeonGreen
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1a-HTLV

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDest-mCherry-N1

    Plasmid

    #31907

    Depositor

    Robin Shaw

    Article

    Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. SEAP (Protein secretion reporter) in pLX317

    Plasmid

    #233290

    Purpose

    SEAP reporter (used for monitoring protein secretion)

    Depositor

    Sefi Rosenbluh

    Article

    Xi et al Proc Natl Acad Sci U S A. 2025 Apr 15;12

    Insert

    SEAP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDule2-Mb haloTyrRS C6

    Plasmid

    #160378

    Purpose

    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyrosine tRNA synthatase
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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