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pFlagCMV2-UbcH13

Plasmid

#12460

Depositor

Dong-Er Zhang

Publication

Zou et al Biochem Biophys Res Commun. 2005 Oct 14.

Insert

Ubc13 (UBE2N Human)

Tags

FLAG

Expression

Mammalian

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-TRAM-CFP

Plasmid

#13027

Depositor

Doug Golenbock

Insert

Toll-like Receptor Adaptor Molecule (TICAM2 Human)

Tags

CFP

Expression

Mammalian

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pTP223

Plasmid

#13263

Depositor

Kenan Murphy

Publication

Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

Insert

Plac-gam-bet-exo

Expression

Bacterial

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX-4T2-14-3-3 tau GST

Plasmid

#13281

Depositor

Michael Yaffe

Publication

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 tao (YWHAQ Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
eGFP STAT1 S727E

Plasmid

#12305

Depositor

Alan Perantoni

Publication

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 S727E (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S727E

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVUTHshGATA1-tTR-KRAB

Plasmid

#11650

Purpose

Tet-regulated (Tet-on) lentiviral vector for shGATA1 (hUbiquitin promoter) - 3rd generation

Depositor

Patrick Aebischer, Didier Trono

Publication

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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1478 pcDNA3 flag HA 14-3-3 beta

Plasmid

#8999

Depositor

William Sellers

Insert

14-3-3 beta (YWHAB Human)

Tags

HA and flag

Expression

Mammalian

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pAWP78-PVCpnf1-16

Plasmid

#198271

Purpose

Unmodified pPVC; contains WT PVC structural/accessory genes

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf1-16

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

Plasmid

#196702

Purpose

Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

Depositor

Karlene Cimprich

Publication

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 wild type

Expression

Mammalian

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

Plasmid

#190181

Purpose

Fluorescent reporter vector to visualize all of cell cycle phases.

Depositor

Toshiro Sato

Publication

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
TagBFP2
hGeminin(1/110)

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDAC627

Plasmid

#195240

Purpose

Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Publication

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro

Expression

Mammalian

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SpCas9 TadCBEd

Plasmid

#193835

Purpose

Express TadCBEd (with SpCas9) in mammalian cells

Depositor

David Liu

Publication

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDd-SpCas9 D10A-2xUGI

Expression

Mammalian

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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VRQR-ABEmax

Plasmid

#119811

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)

Expression

Mammalian

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

Plasmid

#121944

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6 promoters

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSI-Target-AID-NG

Plasmid

#119861

Purpose

Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

Depositor

Osamu Nureki

Publication

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET_RTX_(exo-)

Plasmid

#102786

Purpose

Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

Depositor

Andrew Ellington

Publication

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase with 3'-5' deficient exonuclease

Expression

Bacterial

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKM444

Plasmid

#108319

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor

Expression

Bacterial

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGL3 2 kb prom. CD274

Plasmid

#107003

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Publication

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV.CAG.LSL.tdTomato

Plasmid

#100048

Purpose

AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Publication

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdtomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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