DNA-PKcs T3950D
(Plasmid #83320)

• Purpose: DNA-PKcs T3950A
• Depositing Lab: Katheryn Meek
• Publication: Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. Epub 2006 Dec 11.

Backbone

• Vector backbone: pcmv6
• Backbone size w/o insert (bp): 4665
• Total vector size (bp): 17065
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: see attached propagation instructions. This plasmid is prone to recombination. Transformed E. coli is extremely slow growing; please pick smallest colonies for a test prep, then check for appropriate EcoR1 restriction pattern. Usually, after transformation there will be a population of larger colonies… these are clones that have deletions.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human DNA-PKcs
• Species: H. sapiens (human)
• Insert Size (bp): 12387
• Mutation: Activation loop phosphorylation site 3950 substituted to Asp.
• Entrez Gene: PRKDC (a.k.a. DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350)

Resource Information

• Supplemental Documents:
  • this file describes how the human DNA-PKcs plasmid was originally constructed.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry