|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||155297||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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Vector typeGeneration of SARS-CoV-2-pseudotyped lentiviral particles
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSARS-Cov2 spike_deleted
MutationDeletion in C-term 19 aa of spike
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1_ spike_del19 was a gift from Raffaele De Francesco (Addgene plasmid # 155297 ; http://n2t.net/addgene:155297 ; RRID:Addgene_155297)