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B52 (empty plasmid backbone to express 2 sgRNAs)
(Plasmid #100708)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100708 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    B52 (MLM3636 plasmid backbone modified to express 2 sgRNAs)
  • Backbone size (bp) 2633
  • Modifications to backbone
    The B52 plasmid is a derivative of the MLM3636 construct (Addgene #43860). B52 contains a second sgRNA-expressing cassette, which was PCR amplified from the plasmid eSpCas9 (1.1) (Addgene #71814) and cloned into the MLM3636 plasmid using the restriction enzymes HindIII and XhoI. The final B52 plasmid contains a ColE1 origin, an ampicillin resistance gene and two sgRNA expression cassettes with either BsmBI or BbsI sites for sgRNA cloning.
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter U6 promoters

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    B52 (empty plasmid backbone to express 2 sgRNAs) was a gift from Alberto Ciccia (Addgene plasmid # 100708 ; http://n2t.net/addgene:100708 ; RRID:Addgene_100708)
  • For your References section:

    CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons. Billon P, Bryant EE, Joseph SA, Nambiar TS, Hayward SB, Rothstein R, Ciccia A. Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 10.1016/j.molcel.2017.08.008. 10.1016/j.molcel.2017.08.008 PubMed 28890334