CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons.
Billon P, Bryant EE, Joseph SA, Nambiar TS, Hayward SB, Rothstein R, Ciccia A
Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 10.1016/j.molcel.2017.08.008. PubMed Article
Billon P, Bryant EE, Joseph SA, Nambiar TS, Hayward SB, Rothstein R, Ciccia A
Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 10.1016/j.molcel.2017.08.008. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100708 | B52 (empty plasmid backbone to express 2 sgRNAs) | Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning) |
| 100709 | B52 + SPRTN sgSTOP | B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning) |
| 100710 | B52 + FANCM sgSTOP | B52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning) |
| 100711 | B52 + PARP4 sgSTOP | B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning) |
| 100712 | B52 + CHEK2 sgSTOP | B52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning) |
| 100713 | B52 + TIMELESS sgSTOP | B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning) |
| 100714 | B52 + PIK3R1 sgSTOP | B52 plasmid expressing PI3KR1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning) |
| 100715 | B52 + SMARCAL1 sgSTOP | B52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning) |
| 100716 | B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette) | B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning) |
| 100717 | B270 + SMARCAL1 sgSTOP | B270 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP |
| 100718 | B270 + SPRTN sgSTOP | B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP |