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Programmable RNA editing with compact CRISPR-Cas13 systems from uncultivated microbes.
Xu C, Zhou Y, Xiao Q, He B, Geng G, Wang Z, Cao B, Dong X, Bai W, Wang Y, Wang X, Zhou D, Yuan T, Huo X, Lai J, Yang H
Nat Methods. 2021 May;18(5):499-506. doi: 10.1038/s41592-021-01124-4. Epub 2021 May 3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
171379CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpAExpression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
171380CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpAExpression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
171381CMV-Cas13Y.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpAExpression vector for encoding a human codon-optimized Cas13Y.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
171382CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DRExpression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
171383U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpAExpression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.
171384CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpAExpression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
171385CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpAExpression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Antibodies from Article