PurposeExpression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||171383||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehumanized minidCas13X.1-REPAIRv2
- Promoter CMV, U6
/ Fusion Protein
- HA (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA was a gift from Hui Yang (Addgene plasmid # 171383 ; http://n2t.net/addgene:171383 ; RRID:Addgene_171383)
For your References section:Programmable RNA editing with compact CRISPR-Cas13 systems from uncultivated microbes. Xu C, Zhou Y, Xiao Q, He B, Geng G, Wang Z, Cao B, Dong X, Bai W, Wang Y, Wang X, Zhou D, Yuan T, Huo X, Lai J, Yang H. Nat Methods. 2021 May;18(5):499-506. doi: 10.1038/s41592-021-01124-4. Epub 2021 May 3. 10.1038/s41592-021-01124-4 PubMed 33941935