Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

PCV-Cas9
(Plasmid #123643)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 123643 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    PCV2
  • Alt name
    porcine circovirus2 Rep domain
  • Mutation
    2-116
  • Entrez Gene
    ORF1 (a.k.a. PCV2_gp1)
  • Tags / Fusion Proteins
    • His6 + SUMO (N terminal on backbone)
    • (EAAAK)8 linker (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Cas9
  • Alt name
    S. pyogenes Cas9

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Cas9- from Addgene #62731, PCV2 was synthetic gene
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCV-Cas9 was a gift from Wendy Gordon (Addgene plasmid # 123643 ; http://n2t.net/addgene:123643 ; RRID:Addgene_123643)
  • For your References section:

    Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template. Aird EJ, Lovendahl KN, St Martin A, Harris RS, Gordon WR. Commun Biol. 2018 May 31;1:54. doi: 10.1038/s42003-018-0054-2. eCollection 2018. 10.1038/s42003-018-0054-2 PubMed 30271937