177,635 results
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Plasmid#183775PurposeAAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.DepositorInsertNLS-GFP
UseAAVMutationNAPromoterCAGAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIR-CMV-SCN2A-Variant-1-IRES-mScarlet
Plasmid#162279PurposeEukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSCN2A Variant 1, stabilized (SCN2A Human)
TagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp4551.PromoterCMVAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-HyPer7-MEM
Plasmid#136465PurposeMammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHyPer7
TagsFarnselation tagExpressionMammalianPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CGBE1 (pRZ3885)
Plasmid#140252PurposeCMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteUNG-BE4max(R33A)-ΔUGI
ExpressionMammalianMutationR33A in rAPOBEC1, D10A in Cas9PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyTag003-mKate2
Plasmid#133452PurposeExpresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mKate2
TagsGSGSGS linker and His6ExpressionBacterialPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1 mCherry
Plasmid#128073Purposepuro exchanged to mCherryDepositorInsertmCherry
UseLentiviralExpressionMammalianMutationnoAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VRQR-ABEmax
Plasmid#119811PurposeA-to-G base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
ExpressionMammalianAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE30-His-CKM
Plasmid#124134PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7
Plasmid#121944PurposeCRISPR-Sirius plasmidDepositorInserthU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterU6 promotersAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSI-Target-AID-NG
Plasmid#119861PurposeExpresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
ExpressionMammalianAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET_RTX_(exo-)
Plasmid#102786PurposeExpresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)DepositorInsertreverse transcription xenopolymerase with 3'-5' deficient exonuclease
ExpressionBacterialAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP
Plasmid#111581PurposeAn AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyrTagRFP-T-P2A-post-eGRASP
UseAAVPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM444
Plasmid#108319PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage IntegraseDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
ExpressionBacterialAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3 2 kb prom. CD274
Plasmid#107003PurposeModified Luciferase expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD274 (CD274 Human)
UseLuciferaseExpressionMammalianAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-3xFLAG-TEV-UBE2O
Plasmid#105718PurposeExpresses WT full length human N- terminally tagged UBE2ODepositorAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.LSL.tdTomato
Plasmid#100048PurposeAAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)DepositorInserttdtomato
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
-5.5kb-UCP1-GFP
Plasmid#104585PurposeReporter assay to monitor the transcription activity of the UCP1 promoter by GFP expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUCP1 promoter (Ucp1 Mouse)
TagsTurboGFPExpressionMammalianPromoterUCP1Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLJC5-LAMP1-RFP-2xFlag
Plasmid#102931PurposeLentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)DepositorInsertLAMP1
UseLentiviralTagsmRFP-2xFlagPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSNAP-tag (T7) Vector
Plasmid#101137PurposeExpression of protein of interest with SNAP-Tag in bacterial cellsDepositorTypeEmpty backboneTagsSNAP-tag (SNAP26b)ExpressionBacterialAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
B52 (empty plasmid backbone to express 2 sgRNAs)
Plasmid#100708PurposeEmpty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6 promotersAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by