163,584 results

1. pET MBP His6 LIC cloning vector (2Cc-T)

   Plasmid

   #37237

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-Tet-OsTIR1(F74G)-V5

   Plasmid

   #158664

   Purpose

   Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   Tet-OsTIR1(F74G) AAVS1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   F74G

   Promoter

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTS-hFluc

   Plasmid

   #177716

   Purpose

   Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFluc
   

   Use

   Tags

   Mitochondrial Targeting Sequence MTS SS

   Expression

   Bacterial, Mammalian, and Y…

   Mutation

   Promoter

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Multi-frame tag

   Plasmid

   #128610

   Purpose

   In the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.

   Depositor

   Tim Stasevich

   Article

   Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

   Insert

   MF tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICherryNeo

   Plasmid

   #52119

   Purpose

   a bicistronic IRES-mCherry containing vector for mammalian expression (CMV promoter)

   Depositor

   Dario Vignali

   Type

   Empty backbone

   Use

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. 2Bc-T MBP_BoMoC(ed)_6xH

   Plasmid

   #186461

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

   Insert

   B. mori truncated R2 RT
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

   Plasmid

   #182653

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   mutant eukaryotic release factor 1
   

   Use

   Tags

   HA tag and nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   E55D and Y306A/Y384F (AF) double mutant of Methan…

   Promoter

   CMV and U6

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBT-2

   Plasmid

   #22833

   Depositor

   Ryan Gill

   Article

   Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIP-hPGK-Blast-2A-STING-TurboID

   Plasmid

   #204713

   Purpose

   Expresses C-terminal TurboID tagged human STING for proximity ligation.

   Depositor

   Nir Hacohen

   Article

   Gentili et al Nat Commun. 2023 Feb 4;14(1):611. doi: 1

   Insert

   STING1 (STING1 Human)
   

   Use

   Lentiviral

   Tags

   TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_WNT5A_WT

    Plasmid

    #82240

    Purpose

    Gateway Donor vector containing WNT5A , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    WNT5A (WNT5A Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNC-Cam2304-RNAi

    Plasmid

    #193400

    Purpose

    Nimble cloning-compatible vector for plant RNAi

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-MS2V5-eIF4G delN83

    Plasmid

    #65812

    Purpose

    expression of eIF4G-MS2 fusion.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    eIF4G (EIF4G1 Human)
    

    Use

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    delN83 (functionally behaves as the WT protein)

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI HA NEDD4

    Plasmid

    #27002

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    NEDD4 (NEDD4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. CaVα2δ1

    Plasmid

    #26575

    Depositor

    Diane Lipscombe

    Article

    Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

    Insert

    Cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_YES1-1/1

    Plasmid

    #91439

    Purpose

    Protein expression and purification of human SH3 domain construct YES1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    YES1-1/1 (YES1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-Luc-mCherry-puro

    Plasmid

    #183685

    Purpose

    Lentiviral vector expressing luciferase and mCherry.

    Depositor

    Andrew Kung

    Article

    Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

    Insert

    Luc-E2A-mCherry-T2A-PuroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Human Ubiquitin C (UbC) promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJL1 sortase

    Plasmid

    #106288

    Purpose

    Expresses sortase in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    sortase A pentamutant
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac-M2

    Plasmid

    #135583

    Purpose

    High eukaryotic protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJH2930

    Plasmid

    #175283

    Purpose

    Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a of DAF-16

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    DAF-16a (daf-16 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pges-1

    Available Since

    Dec. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pAWP78-PVCpnf_pvc13-antiHistoneNb

    Plasmid

    #198293

    Purpose

    Produces PVCs retargeted with A9 anti-histone nanobody

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiHistoneNb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge