161,570 results

1. pIB4

   Plasmid

   #25453

   Depositor

   Benjamin Glick

   Article

   Sears et al Yeast. 1998 Jun 15;14(8):783-90.

   Insert

   Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
   

   Use

   Pichia pastoris expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK306

   Plasmid

   #110544

   Purpose

   rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

   Inserts

   PrhaBAD
   yfp
   rhaS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A, PrhaBAD, and kanR promoter (upstream of kanR…

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LifeAct-mNeonGreen

   Plasmid

   #98877

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   LifeAct
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRC_RR_VP1_r1c3

   Plasmid

   #65724

   Purpose

   Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR

   Depositor

   Junghae Suh

   Article

   Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCG_SARS-CoV1 C-V5-tag ORF3a

   Plasmid

   #179969

   Purpose

   Mammalian expression vector for SARS-CoV-1 ORF3a, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   SARS-CoV-1 ORF3a (ORF3a Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCLXEBR-pTF-Ngn3

   Plasmid

   #114307

   Purpose

   TET-inducible expression of the Neugenin 3 transcription factor

   Depositor

   Patrick Salmon

   Article

   Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

   Insert

   Ngn3 (NEUROG3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Epac1 CAAX

   Plasmid

   #118312

   Purpose

   Expression of human Epac1

   Depositor

   Philip Stork

   Article

   Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

   Insert

   Epac1 CAAX (RAPGEF3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C-terminal 20aa of KiRas

   Promoter

   CMV

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJE53

   Plasmid

   #194153

   Purpose

   sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   sgRNA with mrfp control guide sequence
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHen2 Avi-tag-linker-p3

   Plasmid

   #119819

   Purpose

   For cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophage

   Depositor

   Alexander Gabibov

   Article

   Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

   Insert

   linker with Avi-tag and p3
   

   Use

   Tags

   Avi-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102886

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDN34-BiNLS-hYAP1-1delta

    Plasmid

    #169471

    Purpose

    expresses optoYAP in mammalian cells

    Depositor

    Timothy Saunders

    Article

    Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

    Insert

    YAP1-1delta (YAP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid

    #154868

    Purpose

    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

    Depositor

    Ulrik Gether

    Has Service

    AAV Retrograde and AAV8

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin-1

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD21-AI-hLDLR

    Plasmid

    #124964

    Purpose

    Express human LDLR under human APOAI promoter

    Depositor

    Kazuhiro Oka

    Article

    Li et al J Genet Syndr Gene Ther. 2011 Sep 28;2:1

    Insert

    human LDLR (LDLR Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    human APOAI

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSC-NLS-RFP

    Plasmid

    #221813

    Purpose

    Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear localization sequence (NLS)

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    NLS-mCherry
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.GFAP.(cyto).iATPSnFR2.S29W.A95K.HaloTag

    Plasmid

    #209659

    Purpose

    Expression of iATPSnFR2, high affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.S29W.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S29W.A95K

    Promoter

    GFAP

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRsetB-His7tag-Peredox-mCherry

    Plasmid

    #32382

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    His7-Peredox-mCherry
    

    Use

    Tags

    His7tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 myc-RNF114

    Plasmid

    #58295

    Purpose

    mammalian expression of myc tagged RNF114

    Depositor

    Francesca Capon

    Article

    Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

    Insert

    RNF114 (RNF114 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pET51b-His-TEV-SNAP-tag_fast

    Plasmid

    #167271

    Purpose

    Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-FOXO3a WT

    Plasmid

    #8360

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    FOXO3a (FOXO3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGFP-hPMLIII

    Plasmid

    #181903

    Purpose

    Fluorescently tagged PMLIII

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    hPMLIII (PML Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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