165,688 results

1. EF1a-mito-dsRED2

   Plasmid

   #174541

   Purpose

   mitochondria targeted dsRED expressed off of EF1a promoter

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Use

   Lentiviral

   Tags

   dsRED

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Flag-IDH1-R132H

   Plasmid

   #62907

   Purpose

   Expresses R132 mutant IDH1 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

   Insert

   IDH1 (IDH1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deleted Arg132 (R132)

   Available Since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM178

   Plasmid

   #61270

   Purpose

   Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   pLtetO, native, native (respectively)

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP scFv [N86/8]

   Plasmid

   #199429

   Purpose

   Mammalian expression of GFP scFv. Derived from hybridoma N86/8.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targetingGFP (Aequorea victoria)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-Tight-Puro FLAG-SMAD4 TetON

   Plasmid

   #107129

   Purpose

   Inducible expression of Flag-tagged SMAD4 in mammalian cells

   Depositor

   Joan Massague

   Article

   David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

   Insert

   SMAD4
   

   Use

   Lentiviral

   Tags

   FLAG

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. SuperNova Green/ pRSETB

   Plasmid

   #124839

   Purpose

   bacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Riani et al BMC Biol. 2018 Apr 30;16(1):50. doi: 10.

   Insert

   SuperNova Green
   

   Tags

   No tag

   Expression

   Bacterial

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-M2

   Plasmid

   #135583

   Purpose

   High eukaryotic protein expression shuttle vector

   Depositor

   Xiao-Wen Cheng

   Article

   Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

   Type

   Empty backbone

   Use

   Transfer vector

   Expression

   Insect

   Promoter

   polyhedrin of AcMNPV

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. NET5s pEGFP-N2 (1154)

   Plasmid

   #62034

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET5s (TMEM201 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   short form

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

   Plasmid

   #140220

   Purpose

   Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   dLbCpf1
   

   Use

   CRISPR

   Tags

   miniVPR-mCherry

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   PGK

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-BirA*-dCas9-eGFP

    Plasmid

    #84779

    Purpose

    Expresses BirA*-dCas9-eGFP in mammalian cells.

    Depositor

    Heinrich Leonhardt

    Article

    Schmidtmann et al Nucleus. 2016 Sep 27:0.

    Insert

    BirA*-dCas9-eGFP
    

    Use

    CRISPR

    Tags

    cMyc

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    July 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-TAF1

    Plasmid

    #65395

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    TAF1 (TAF1 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2T-CMV-ABEmax-BlastR

    Plasmid

    #152989

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    ABE7.10-nSpCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. N-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58693

    Purpose

    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    

    Use

    AAV and CRISPR

    Tags

    3xFlag, GyrA Nsplit Intein, and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-FOXL2-cterm

    Plasmid

    #192893

    Purpose

    Expresses Cas9 and sgRNA targeting the C-terminus region of FOXL2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOXL2 C-term sgRNA (FOXL2 Synthetic)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18T-mini-Tn7T-Gm-Pc-DsRed-Express2

    Plasmid

    #111260

    Purpose

    mini-Tn7 vector for insertion of DsRed-Express2 at bacterial attTn7 site

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    DsRed-Express2
    

    Expression

    Bacterial

    Promoter

    Pc

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET LIC cloning vector (2A-T)

    Plasmid

    #29665

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pprm1-luciferase; his-3

    Plasmid

    #137728

    Purpose

    Communication-activated luciferase in Neurospora crassa

    Depositor

    Louise Glass

    Article

    Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi

    Inserts

    Luciferase
    Pprm-1 Promoter
    

    Use

    Luciferase; Neurospora crassa expression

    Mutation

    Codon-optimized for N. crassa

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC-1-AtUbi10-6

    Plasmid

    #197700

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AtUbi10 promoter
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLIK-IDH1-FLAG

    Plasmid

    #66802

    Purpose

    Tet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vector

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. hPlekhm1-dsRedM

    Plasmid

    #73592

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Tags

    dsRed

    Expression

    Mammalian

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only