174,421 results

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pCL-MFG-BRCA1

Plasmid

#12341

Depositor

Inder Verma

Article

Ruffner et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

BRCA1 (BRCA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pL3-TRE-LucGFP-2L

Plasmid

#11685

Depositor

Geoff Wahl

Article

Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Insert

Luciferase-EGFP

Use

Cre/Lox

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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1481 pcDNA3 flag HA 14-3-3 zeta

Plasmid

#9002

Depositor

William Sellers

Insert

14-3-3 zeta (YWHAZ Bovine)

Tags

HA and flag

Expression

Mammalian

Mutation

T226A

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pCINeo-myc-Itch (C822S)/2860

Plasmid

#11428

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Itch (Itch Mouse)

Tags

myc

Expression

Mammalian

Mutation

cysteine at 822 mutated to serine

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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1435 pSG5L Flag HA

Plasmid

#10791

Purpose

Mammalian expression empty backbone.

Depositor

William Sellers

Type

Empty backbone

Tags

Flag and HA

Expression

Mammalian

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo smallT

Plasmid

#8583

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

Plasmid

#196702

Purpose

Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 wild type

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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p4xmts-mScarlet3_N1

Plasmid

#189774

Purpose

Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

4xmts-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

Plasmid

#188778

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMG36E

Plasmid

#133853

Purpose

Expression vector

Depositor

Jan Kok, Gerard Venema

Article

van de Guchte et al Appl Environ Microbiol. 1989 Jan;55(1):2

Type

Empty backbone

Expression

Bacterial

Promoter

P32

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Hs.cargo(Cre)

Plasmid

#174863

Purpose

Expresses human Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by HsPeg10 UTRs

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS007: PercevalHR ATP/ADP sensor (cytosolic)

Plasmid

#163061

Purpose

PercevalHR ATP/ADP sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

PercevalHR

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF8

Plasmid

#141278

Purpose

Gateway-compatible Entry vector, with insert of ORF8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF8 (ORF8 SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

Plasmid

#134867

Purpose

Expresses EGFP-tagged nuclear envelope marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pC0079 RESCUE-S

Plasmid

#130662

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b-ADAR2dd

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Mito-mEosEM

Plasmid

#132706

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mitochondria targeting sequence

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP670nano

Plasmid

#127434

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

LifeAct-miRFP670nano

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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Halo-KDEL

Plasmid

#124316

Purpose

Expresses ER specific HaloTag protein

Depositor

Jin Wang

Article

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Tags

KDEL Seq

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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TEVSH

Plasmid

#125194

Purpose

His-tagged TEV protease with solubility increasing mutations

Depositor

Helena Berglund

Article

van den Berg et al J Biotechnol. 2006 Feb 10;121(3):291-8.

Insert

TEV_SH

Tags

V5 His-tag

Expression

Bacterial

Promoter

T7lac

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAJ075_hsCRBN

Plasmid

#124214

Purpose

Insect cell expression vector for His6 tagged hsCRBN

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

Cereblon (CRBN Human)

Tags

His6-TEV

Expression

Insect

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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