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pcDNA3.1 FLAG-YY1

Plasmid

#104396

Purpose

FLAG-YY1

Depositor

Richard Young

Publication

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

YY1

Tags

FLAG

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDG461

Plasmid

#100902

Purpose

SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

Depositor

Paul Thomas

Publication

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG and T2A-EGFP

Expression

Mammalian

Mutation

D10A mutant converts to Nickase

Promoter

CBh and U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Tau (wt) -VC

Plasmid

#87369

Purpose

expresses hTau fused to the C-terminal part of Venus

Depositor

Tiago Outeiro

Publication

Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAPT (MAPT Human)

Tags

c-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHR-tdMCP-YFP

Plasmid

#99151

Purpose

Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

Depositor

Ron Vale

Publication

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

Dimeric MS2-coat binding protein

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Promoter

SFFV

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRN11

Plasmid

#84455

Purpose

shuttle plasmid for sarA P1-mCherry expression

Depositor

Reindert Nijland

Publication

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LVXN-Neo-NSD2

Plasmid

#86010

Purpose

express wild-type NSD2 in mammalian cells

Depositor

Darrin Stuart

Publication

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 (NSD2 Human)

Use

Lentiviral

Tags

Flag tag D Y K D D D D K

Expression

Bacterial

Mutation

K1002R (please see depositor comments below)

Promoter

CMV

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pY026

Plasmid

#84741

Purpose

Expresses huAsCpf1 and crRNA guide

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

huAsCpf1

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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hSyn1-sypHy-RGECO

Plasmid

#84078

Purpose

Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis

Depositor

Juan Burrone

Publication

Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sypHy-RGECO

Expression

Mammalian

Promoter

hSyn1

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-ALK5 T204D

Plasmid

#80877

Purpose

mammalian expression of constitutively active ALK5 T204D

Depositor

Aristidis Moustakas

Publication

Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ALK5 (TGFBR1 Human)

Tags

HA

Expression

Mammalian

Mutation

T204D (constitutively active)

Promoter

CMV

Available since

Aug. 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pUCP20T-E2Crimson

Plasmid

#78473

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Publication

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

E2-Crimson

Expression

Bacterial

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLgw V5-EcoDam

Plasmid

#59210

Purpose

Mammalian DamID control lentiviral vector for expression of V5-Dam only

Depositor

Bas van Steensel

Publication

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Insert

EcoDam

Use

Lentiviral; Damid

Tags

V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter and heat shock minima…

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA3-HA-Akt1

Plasmid

#73408

Purpose

Expresses human AKT1 with a N-terminus HA tag

Depositor

Jie Chen

Publication

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pEGFP-Anillin

Plasmid

#68027

Purpose

Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

Depositor

Michael Glotzer

Publication

Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

Insert

Anillin (ANLN Human)

Tags

eGFP

Expression

Mammalian

Mutation

silent mutations at 798 5'-TGCC TCTTTGAATAAA…

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mRFP-FKBP12

Plasmid

#67514

Purpose

FKBP12 only

Depositor

Tamas Balla

Publication

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP12 (FKBP1A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pYLsgRNA-AtU3d

Plasmid

#66200

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

AtU3d

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFUGW-hGtACR1-EYFP

Plasmid

#67795

Purpose

Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

Depositor

John Spudich

Publication

Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

Insert

hGtACR1

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMitoLOC

Plasmid

#58980

Purpose

Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

Depositor

Markus Ralser

Publication

Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

Inserts

mCherry
GFP

Tags

preCOX4 and preSU9

Expression

Yeast

Promoter

ADH1 and MET17

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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GFP-GCN5

Plasmid

#65386

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GCN5 (KAT2A Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pU6a:sgRNA#2

Plasmid

#64246

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Publication

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCAG-VSVG

Plasmid

#64084

Purpose

Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene

Depositor

Ian Wickersham

Insert

VSV glycoprotein

Expression

Mammalian

Promoter

CAG

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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