Addgene

Showing: 6021 - 6040 of 157643 results

Results per page:

Sort results by:

APEX2-V5-G3BP1_pTRE-G418

Plasmid

#202013

Purpose

expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

APEX2-V5-G3BP1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pTRE-Tight

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-ack

Plasmid

#62654

Purpose

Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

exo, beta, gam, sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 TFAM-mScarlet

Plasmid

#129573

Purpose

Expression of mScarlet fused TFAM

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

TFAM (TFAM Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-EN1201

Plasmid

#92144

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Pet28a_Human_H4

Plasmid

#42633

Depositor

Joe Landry

Insert

Human H4 (H4C1 Human)

Use

Tags

Untagged

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223_SUV39H1_WT

Plasmid

#82236

Purpose

Gateway Donor vector containing SUV39H1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SUV39H1 (SUV39H1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CW3SL-EGFP

Plasmid

#61463

Purpose

AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

Depositor

Bong-Kiun Kaang

Article

Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB80 (R26-CreER)

Plasmid

#12168

Depositor

Tyler Jacks

Article

McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Rosa26 CreER^T2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Mutation

Promoter

MHCK7

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-cmyc

Plasmid

#16011

Purpose

mammalian expression of human cmyc

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

cmyc (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Flag-humanTRAF6 (1093)

Plasmid

#66929

Purpose

Mammalian Expression of TRAF6

Depositor

Michael Karin

Insert

TRAF6 (TRAF6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
RCAS GFP (CT#22)

Plasmid

#13878

Depositor

Connie Cepko

Article

Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

Insert

GFP

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV JMJD3 mutant

Plasmid

#21214

Depositor

Paul Khavari

Article

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

H1390A

Promoter

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

Promoter

Simian CMV IE94

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
KA0637_pPBCAG-rtTAM2-IN

Plasmid

#124166

Purpose

piggyBac-based expression of rtTA-M2

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

rtTAM2-IRES-Neo

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9-DNMT3A-PuroR_hNT

Plasmid

#71830

Purpose

Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

Depositor

Vlatka Zoldos

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

Non-targeting sgRNA human (DNMT3A Human, S. pyogenes, Synthetic)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

U6

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.(mito).cpSFGFP.HaloTag

Plasmid

#214925

Purpose

Expresses mitochondrially targeted non-responsive control

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).cpSFGFP.HaloTag

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQUAST

Plasmid

#24349

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

5xQUAS-Pmin promoter

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 12, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK462 (Hygro-P2A-mAB)

Plasmid

#214388

Purpose

mAID-BromoTag for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

Hygro-P2A-mAID-BromoTag

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-Sac-Abeta(M1-42)

Plasmid

#71875

Purpose

Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-42) (APP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge