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Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells.
Varnai P, Thyagarajan B, Rohacs T, Balla T
J Cell Biol. 2006 Nov 6;175(3):377-82.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
67514mRFP-FKBP12FKBP12 only
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67515mRFP-FKBP12-5ptpase enzymeRecruitable human type IV 5-phosphatase enzyme
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67516mRFP-FKBP12-5ptpase domainRecruitable human type IV 5-phosphatase domain (214-644)
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67517PM-FRB-CFPFRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)
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67518PM2-FRB-CFPFRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)
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Antibodies from Article