PurposeFRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67517||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
SpeciesH. sapiens (human)
MutationFRB domain only (residues 2019–2114)
Entrez GeneMTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
- Promoter CMV
/ Fusion Proteins
- PM tethering sequence (MLCCMRRTKQVEKNDDDQKI) from human GAP43 (residues 1–20) (N terminal on insert)
- HA (C terminal on insert)
- CFP (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PM-FRB-CFP was a gift from Tamas Balla (Addgene plasmid # 67517 ; http://n2t.net/addgene:67517 ; RRID:Addgene_67517)
For your References section:Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells. Varnai P, Thyagarajan B, Rohacs T, Balla T. J Cell Biol. 2006 Nov 6;175(3):377-82. 10.1083/jcb.200607116 PubMed 17088424