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PM-FRB-CFP
(Plasmid #67517)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67517 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 67517-D50 50 μg of DNA in Tris buffer $495
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DNA 67517-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    CFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mTOR
  • Species
    H. sapiens (human)
  • Mutation
    FRB domain only (residues 2019–2114)
  • Entrez Gene
    MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
  • Promoter CMV
  • Tags / Fusion Proteins
    • PM tethering sequence (MLCCMRRTKQVEKNDDDQKI) from human GAP43 (residues 1–20) (N terminal on insert)
    • HA (C terminal on insert)
    • CFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PM-FRB-CFP was a gift from Tamas Balla (Addgene plasmid # 67517 ; http://n2t.net/addgene:67517 ; RRID:Addgene_67517)

  • For your References section:

    Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells. Varnai P, Thyagarajan B, Rohacs T, Balla T. J Cell Biol. 2006 Nov 6;175(3):377-82. 10.1083/jcb.200607116 PubMed 17088424
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